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It Only Works Once

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...and if it fails, too bad. You only get the one shot.
It Only Works Once is a trick or technique which is completely useless in the following episodes. It could be something where you literally have only one shot, like a Forgotten Superweapon or other Applied Phlebotinum that cannot be replaced once it's used up. It might be a tactic that relies on the element of surprise, like an attempt to exploit an Achilles' Heel vulnerability that the enemy will correct or at least guard more carefully once it's brought to his attention.
Advertisement:propertag.cmd.push(function() { proper_display('tvtropes_mobile_ad_1'); })Reversed, it is a common trope of cartoon series. The Big Bad will come up with some undefeatable plan that almost works save for some streak of luck on the part of the heroes (see the Road Runner shorts for some especially lucky breaks), but will never think of trying it again after being foiled the first time.
Occasionally a special attack that Only Works Once will end up being used in The Worf Barrage.
When you're getting near the end of a show or film and you know the trope will be averted because it's near the end and not a tragedy work, you're being Spoiled by the Format.
Never Recycle Your Schemes is a subtrope. Compare So Last Season, Forgotten Phlebotinum, Holding Back the Phlebotinum, No Plans, No Prototype, No Backup. See also Adaptive Ability, Reed Richards Is Useless, and the Disposable Superhero Maker. Most Wave Motion Guns apply a limited form of this trope by making its use prohibitively slow and energy-intensive, but the usual dramatic application is rather different. Often overlaps with Death-or-Glory Attack.
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Jimmy Olsen once discovered Superman's secret identity. At the end of the story, Superman used a combination of heat- and microscopic-visions, focused through a space jewel and directed through Jimmy's eyes, to burn out the portion of Jimmy's brain which held that piece of information. Superman lampshaded the trope by telling Jimmy that he could only perform this trick once: if he ever tried it again, he'd short-circuit his vision powers.
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In Gamera vs. Barugon, the humans goad Barugon into firing his rainbow beam at a giant mirror, which bounces back and knocks him down. When the humans try it again, Barugon realizes what is going on and instead attacks them physically. He doesn't use his rainbow beam for the remainder of the film, except once out of desperation when Gamera kills him.
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The Gravity Cannon, from Zoids: Chaotic Century, only had three shots available, one of which had to be a test-fire. It didn't work. A fourth shell is later created, it still doesn't work.
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In Star Ocean: The Second Story, Claude uses his phaser beam (which the planet's locals call a "sword of light") to dispose of a beast attacking Rena. When he gets to the second town, he uses the phaser to break down a door, and it promptly (and inconveniently) runs out of power, and can never be used again.
Depending on how much you've spammed it to level yourself up first, it may or may not be justified. The anime changes it so that it doesn't run out of power, but rather, Alan damages it so that it can't fire.
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Wonder Woman (1987): During their fight against the Sangtee Empire the rebels use the trick of leaving one of their ships floating disabled with breaches in the hull near a shipping lane, with the crew inside playing dead in spacesuits to take advantage of the empire's salvaging practices in order to get the jump on them. The next time the empire sees a damaged and disabled rebel ship they instead destroy it from afar. The rebels were using the ship as a different kind of trap this time and were able to hack the empire's ships after the destruction of the abandoned ship alerted them to their whereabouts.
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All-New Ultimates: There's a giant in town, but Kitty can not repeat the trick she used against Galactus. She needed helpt from SHIELD to be turned into a giant, and there's no SHIELD anymore.
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In Samurai Jack, Aku manages to fool Jack by transforming into a beautiful woman named Ikra and feeding him a sob story to gain his trust, which results in Aku trashing a portal to the past and taunting Jack about it. The second time Aku tries this trick, however (this time as a Yoda-ish old master), Jack immediately sees through it: he Bluffs The Imposter, turns the plan against Aku, and nearly takes him out once and for all.
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The Astro City "Dark Ages" story arc featured the Innocent Gun, a powerful mystic superweapon that was left behind by alien Precursors to be used to protect humanity from a vaguely unspecified future threat, and was crafted so that it could only be used once. Unfortunately, The Lancer ended up using it against another Big Bad, tearing a hole in reality and leaving the gun unavailable for future use.
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Disgaea: In the second chapter, Flonne summons a dragon to attack you, announcing that because she has to use a special item, she can only do it once - thus, after she joins your party, there will be no dragon-summoning.
This can be partially averted... by capturing the dragon she summons, allowing YOU to summon the dragon whenever you want!
And then it's entirely averted... In Disgaea 4, where both Archeangel Flonne and Fallen Angel Flonne have it as a move, with the same incantation. The former summons a normal dragon while the latter summons a zombie dragon.
For a more literal interpretation of this trope, Bonus Boss Pringer X is this trope. Any special attack used against him will only damage him once. If you're fighting multiple Pringers, they all become to immune to the special move.
Tyrant Overlord Baal, as he usually does within the series, took this trope Up to Eleven in his battle in Dimension 2. Even normal attacks can be no-sold by him after he takes a hit, thus demanding your character(s) to have all of the weapon skills in order to win.
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Ranma ½:
In a similar instance to the Saint Seiya example above, an OVA pitted (female) Ranma and Akane versus two powerful sisters (never happened in the manga). After getting creamed in their first match, the two leads train for a rematch. The sisters' ordinary attacks then prove ineffective against Akane (who has trained up to their level) and their special skills are useless against Ranma (who explicitly says, "Your wind attack ain't gonna get me twice! I've seen it once and it's easier to avoid it!")
Turning the trope on the villain's favor, the manga confronted Ranma with the Musk Prince Herb, descendant of a dragon and master of chi techniques. When Ranma tried to use the overwhelmingly powerful Hiryu Shoten Ha technique on him, Herb recognized it and easily dodged it, since he had seen it many times before in the past and it was arguably part of his education. This is later turned around on Herb when Ranma tries the technique again, mocking her that it won’t work since he’s dodged and countered it before. Ranma counters that Herb’s dodge move won’t work this time as she had upgraded the technique within the battle into the Hiryu Korin Dan. This was the same problem with Happōsai, who had once been on the receiving end of this technique (from a young Cologne) and already knew how to thwart it.
Even further: when Ranma used the Parlay du Fois Gras technique on Picolet Chardin, it only worked for a few instants; both he and his teacher recognized the technique and immediately used a variety of defenses. Although Ranma tried to use new variations to get around these counters, each only worked once, until she was left with no way to attack her opponent.
Shampoo once offered Ranma a packet of bath powder that would nullify his curse in exchange for a date. She learns soon after that the powder works only once, but leaves that part out when Ranma finally gets the powder.
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An explicit theme of Fine Structure. Any given superscience technique will only work briefly before the Imprisoning God notices and changes the physical laws of the Universe to make it impossible.
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Bleach:
The Kido Cannon in Bleach: Memories of Nobody burns itself to ash after being fired only once.
The series has shown a couple of examples where a character can enter a Super Mode at the cost of losing all of their powers when it wears off. Naturally, it also provides ways to regain their powers.
There's also Muramasa, main antagonist of the anime filler Zanpakutō Rebellion arc. Muramasa is a Zanpakutō spirit whose power is bringing other Zanpakutō spirits to life and turning them against their masters. He does this by tapping into their most suppressed instinct and using it to control them. The only clear way to break the spell is to defeat the Zanpakutō spirit, but if that happens, Muramasa's power won't be able to affect it a second time.
This is what makes defeating Askin so difficult. Because he has the power to control his and others' resistance to anything he's experienced and absorbed once before, if it didn't kill him the first time (and he's really hard to kill), it won't affect him at all the second.
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In an early episode of Pee-wee's Playhouse involving a thunderstorm, Randy shows Pee-Wee how to prank call someone by calling up a woman on the picture phone and asking "Is your refrigerator running?". When it's Pee-Wee's turn to do a prank call, he calls up the exact same person with the exact same joke. She obviously doesn't fall for it the second time.
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The Legend of Zelda: Breath of the Wild:
Played with and zig-zagged when it comes to the plan of taking Calamity Ganon down. In the distant past, Calamity Ganon appeared and the Guardians and Divine Beasts played a vital role in defeating him, with the Hyrulian princess and a heroic knight eventually sealing him away. When a prophecy foretold the return of Calamity Ganon, King Rhoam had the Guardians and Divine Beasts excavated to recreate the circumstances of the ancient battle. Unfortunately, Calamity Ganon had learned how he was previously defeated and corrupted the Guardians and Divine Beasts with malice and turned them against Hyrule. But since Calamity Ganon is defeated by Link fighting him and Zelda sealing him away with her divine power, the plan does end up working more than once.
During the fight with Maz Koshia at the end of "The Champions' Ballad" DLC, Link can use some Mighty Bananas as a Delicious Distraction, though the boss will only fall for it once per fight.
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In Ace Academy, it turns out that the overdrive function of the main character's GEAR only activated once because it was a one-time debug mode feature. After finding this out, Valerie decides to program it so that the main character can activate it on demand.
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Salem: For some reason, after Mercy's snake familiar is exorcised from her, she's now capable of staying in control of herself after Mary Sibley tries to put it back.
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The television series V (1983) was premised on this. The red dust which was all-powerful in stopping the Visitors at the end of the V miniseries was suddenly found to be ineffective in warmer climates. Also, re-application of the red dust wasn't an option since it was found that repeated exposures to the virus could be deadly to Earth lifeforms too over time (as opposed to instant death for the Visitors).
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In On Basilisk Station, we are introduced to the grav lance, which allows even a light cruiser to take down a superdreadnought's sidewall, but has a very short range, a quarter that of standard energy weapons. Getting it to work requires immense sneakiness and the enemy not looking out for it. It works the first time because the OpFor was not expecting it, but in subsequent exercises the other side knows how to look out for its attempted use. It is these repeated failures that get Honor the eponymous Reassigned to Antarctica. In the final battle against a Havenite Q-ship that outmasses even a super-dreadnought, she manages to pull off another successful use.
This is stated by Harrington to her students concerning her escape from Hades: the stunt she pulled to take out the State Sec convoy coming to retake the planet had so much that could have conceivably gone wrong, depended on the enemy being extremely sloppy in monitoring their sensors, relied on having detailed information regarding their approach that would be near-impossible to have in any other situation, and she had absolutely no other choice except to try it.
Subverted in Storm From the Shadows, as the Mesan plan involving New Tuscany is, and is lampshaded as, a repeat of the plan used in Shadow of Saganami. However, the second attempt succeeds in its intended goal of provoking a war between Manticore and the Solarian League.
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Subverted in The Simpsons where they try to get Maggie out of a locked bathroom. Homer tries a coat hanger then gives up. Lisa tries it again and successfully unlocks the door.
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iCarly: In his first episode, Carly, Sam, and Freddie realize that all they really need to do to stop Nevel is tattle on him to his mom (since he is only 11). This is handwaved in his next appearance where they mention that his mom is going out of town and will be unreachable for a while. After this, it is never brought up again.
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A more straightforward Looney Tunes example would be the Bugs Bunny / Daffy Duck cartoon "Show Biz Bugs" in which he is trying to go one-up on Bugs in their stage act. When none of his tricks impress the audience, he desperately tries his ultimate trick: downing several kinds of volatile explosives and then swallowing a lit match ("Girls...better hold onto your boyfriends!"). The trope title is obviously the punchline to this gag. The same gag was also used in "Curtain Razor" with a wolf trying to impress Porky Pig (which is a talent impresario in this short) with his act. In the end, the wolf gets his chance after several attempts, then doing the trick aforementioned, and as expected, the same punchline (kinda) is delivered.
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This was actually used in the pilot of Justice League, when Flash asks Martian Manhunter why he doesn't use the nerve gas he used to defeat the Imperium before:
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Fallout: Equestria: Alicorns have a whole suite of powerful magical abilities, plus a Hive Mind. Therefore, any time Littlepip finds a way to trick around their impregnable shields, next time they're ready for it. Stealth is the preferred method of dealing with them, but it's not always an option since they're often the ones ambushing her.
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Tengen Toppa Gurren Lagann. During their first encounter, Viral makes a point of telling Kamina sneak attacks only work once.
He's later proven wrong. By the Lagann Impact, no less.
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In I Shall Wear Midnight, Tiffany is told that while the Cunning Man has been defeated before, learning how it was done won't help her and she'll need to devise her own strategy against him. The reason is that he won't fall for the same trick twice - he's cunning, after all.
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Naruto:
Izanagi, one of the Sharingan's powers lets the user cast an illusion upon himself making reality a dream and thus letting them escape life-threatening situations. However, the eye shuts permanently after it is used once. The same apply to the techniques counterpart, Izanami, that locks the victim in an endless loop.
Deidara's "ultimate art" C0 involves using himself as a bomb. Too bad for him, Sasuke had just made in advance an ultimate escape plan for such an occasion.
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In Digimon Xros Wars there's the digimemories which have the data of legendary Digimon, and can summon them to use their abilities. But they can only be used once for each zone.
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Human transmutation in Fullmetal Alchemist. That's because you have to give up your ability to use alchemy to do it, plus the person who you're bringing back has to be trapped at the gate (and thus not actually dead), plus your souls have to be linked so that you can return through their gate once you've given up your own.
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The Martian Manhunter once beat Despero with his innate Martian power to make someone believe that they're experiencing their most fervent desire. He'd never mentioned this power before and would never use it again, because, he explained, using it was so stressful that any one Martian could only do it once in their life. (Focusing on the pathos of his decision to use a power that his culture considered the ultimate gift to give a loved one to defeat a rampaging villain helped gloss over the Ass Pull.)
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The original 40s Captain Marvel once fought a character that could cast any spell - once. He also had one, and only one, weakness. Their fights consisted of the two of them flipping through notebooks that listed everything they had tried so far, trying to come up with strategies.
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RWBY: At one point Ruby summons Jinn in order to use her Time Stands Still ability to get a moment to focus on her Silver Eyes instead of asking her a question. Jinn admonishes Ruby that this is not an acceptable use of her power, but lets her get away with it just this once because she respects Ruby's quick thinking and audacity — and of course, it wouldn't be either of those things to try the same trick again.
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The Amazing Spider Man 2: During his first battle against Electro, Spider-Man hits him with water, defeating him. By the time Spidey uses the trick again, Electro had already figured out a way to create a barrier to protect himself.
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Early in the Shannara series, Allanon mentions that certain powerful works of magic can only be performed once and then never again. This is the reason why the heroes of the various books have to spend so much time hunting down or protecting one MacGuffin or another - if, e.g., the Sword of Shannara goes missing, you can't just forge a new one to replace it.
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The omnipotent Oracle from Aladdin and the King of Thieves is "bound by the Rule of One: one question, one answer". She knows everything and will answer one question to any person who asks. After, she will never answer another of their questions (much to Iago's frustration).
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Yandere Simulator: Certain actions (mostly murder) you can only get away with once, because everyone will be on the alert the next time. Go on a masked murder spree? Masks are banned from school. Kill a student council president? The School Atmosphere will drop to its lowest (making everyone more watchful), and metal detectors and security cameras will be there to cause problems. Each rival also has a person-specific murder method, that you only get one chance at as they won't be in position at any other time (i.e. Kizana is killed at the play she's acting in).
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In an old Marvel Team-Up issue, Black Panther concocted a super strong web fluid to hold Stegron, a villain that had previously broken out of Spider-Man's webs. It was stated that the new serum was very unstable, and Black Panther himself warned Spider-Man that it could only be used on this one occasion.
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Reaper had Sam, the main character, use vessels to catch escaped souls from hell. Each week's vessel was different, being an item related to what the escaped person had been sent to hell for.
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In Cwen's Quest mysterious life slave Riddly Lancer defeats a huge dragon with an equally huge DBZ style blast. Next chapter when asked to do it again against to defeat the next bad guy he simply reports he can't do it again. Luckily he apparently has a few other skills.
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In the New Jedi Order series of Star Wars novels, someone comes up with the brilliant idea of using the Centerpoint Station superweapon to destroy a Yuuzhan Vong fleet. Unfortunately the heroes get righteous on the guy trying to fire the weapon, and cause him to misfire. The weapon destroys a major Galactic Alliance fleet, and Centerpoint powers down, unable to be used ever again.
A milder version in the same series, Jedi Knight Corran Horn has a "dual-phase" lightsaber, which allows the blade to extend in length. At one point, he surprises one of a pair of Yuuzhan Vong warriors by suddenly extending the blade, impaling him. He monologues to the other warrior (who may or may not have been able to understand him) that he knew he'd only be able to use the trick once, and chose to use it on the obviously less-skilled warrior, since he'd be more likely to fall for it.
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In Romance of the Three Kingdoms Zhuge Liang used the Empty Fort Strategy, but then claimed it to be this trope. Specifically, he orders his few hundred soldiers to dress as civilians and all the city gates to be thrown open. Sima Yi is wary of Zhuge Liang's strategic brilliance and assumes it to be a triple bluff as Zhuge Liang's plans never leave his army open to this kind of risk.
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In Fairy Tail: 100 Years Quest, Erza states that the weakness of hypnotism-based magic is that once a person knows how the specific spell works on the target, they can counter further usages of it, going so far as to call said weakness "understanding." She proves it by No Selling Kyria's "cutting mentality" spell in their rematch after being subjected to and recovering from its effects from their first fight.
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Deadpool 2: Deadpool asks Cable why he can't use his time travel device to just keep bouncing around time until he gets everything right. Cable says his device only has enough charge left for one more jump, which he needs to get back home.
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Supernatural: In season 7, Sam and Dean eventually acquire a Villain-Beating Artifact that can be used to kill an otherwise Nigh Invulnerable Leviathan, but it can only be used once. They decide to target their leader Dick Roman, hoping that his army will collapse without him. When the weapon is finally used, it sucks both Dean and Castiel along with Roman into the monster afterlife.
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In an episode of Hot Wheels Battle Force 5, the team needs to head to a nanotech stabilizer and can't afford to go through a Battle Zone first, so Saige reveals a crystal that lets them skip the Battle Zone and go straight to their destination. When asked why they don't always use such a crystal, she reveals that she only managed to obtain one, thus limiting its use.
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Whateley Universe: A few devises work like that. The Psi-grenade, cannot be recharged after its been detonated and Mega-Death's Force Field Disruptor burns itself out after its been used. But multiples of each can be made.
This is a semi-common trait of devisor items. They specifically cannot be replicated, because they depend upon the mutant's reality warping power. So each individual black hole grenade is its own unique item. This has led to a kind of disconnect between Devisors and Gadgeteers. The former sells you an ultra-powerful weapon you can use precisely once. The latter sells you a slightly less powerful weapon you can actually reproduce.
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In the first of the films featuring Mechagodzilla, Godzilla was able to defeat his Evil Knockoff by tearing off his head, removing the controls and cameras that let the mechanical monster keep moving. The second time they faced off, Godzilla repeated the move and was shocked when a second, smaller head emerged from the gap and blasted him with lasers.
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Mojo Jojo tries to avert this in an episode of The Powerpuff Girls by trying an old plan a second time, thinking he's taken precautions against what ruined the plan the first time. The girls defeat him anyway, showing that maybe it's best to turn your back on plans that have already failed once after all. Considering that his precaution was to not turn the girls into dogs, thus preventing them from biting his butt (now protected by a metal plate, one would think that doing either of those things cancels out the usefulness of doing the other, but whatever) and making him break the magic idol he was using, they weren't very good precautions. He forgot that, since the girls aren't transformed, they can foil his plans in the same way they foil all his plans; by beating him up.
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Owing to a programming error, the Dragon Zombie of Final Fantasy VII will cast its ultimate spell "Pandora's Box" at you only once - after that, no enemy will ever attempt the spell on you again (unless you start the game over). Your party can learn the attack for themselves, but if you didn't have an Enemy Skill material on them at the time, it's gone for good.
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Inuyasha:
Practically speaking, Miroku's Wind Tunnel. It should be able to instakill every enemy the troupe comes across due to its ability to suck everything into the pocket dimension in his hand, but it takes Naraku one encounter to learn how to neutralize it... which makes sense, considering he's the one who put it there in the first place.
Later, Sesshomaru discovers the hard way that the Tenseiga can only bring a single person Back from the Dead once.
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Tony's badass wrist-lasers in Iron Man 2. Justified when we see Tony eject a pair of smoking doohickies from his gauntlets after firing the lasers. The single shot clearly burns the doohickies out, leaving him physically unable to use the weapon again at the time. Since he doesn't grab a new pair of doohickies out of a storage compartment, we can assume that re-readying the lasers requires more than a simple doohicky swap. Doohicky. Lampshaded by Tony himself when Rhodey says he should have led with that: "It's a one-off."
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XCOM: Enemy Unknown
Rockets. Since the Heavy Class is carrying a light machine gun (the heaviest weapon in the game), he is already very burdened, so he can only carry one rocket, with no way to resupply during a battle. Well, technically, if he takes the right promotion perks, he can use two rockets and a special shredder rocket (which causes less damage but inflict a Damage-Increasing Debuff), still, with a maximum of three rockets, make them count.
All active items work similarly. The medikit gets one use (three with a Field Medic Support), four when he gets Deep Pocket in Enemy Within), grenades can only be used once (twice with the Grenadier Perk from the Heavy, or the Deep Pocket support ability in Enemy Within), the Arc Thrower can only be used twice (thrice with the Support ability Deep Pockets), the support Smoke Grenade can only be used once, twice with the Smoke And Mirrors ability (thrice in Enemy Within). It had to be noted it applies only during missions, as the squad gets resupplied for free between missions.
In XCOM: Enemy Within, there is a scripted event involving an alien attack on your base (if you lose this mission, it's Nonstandard Game Over for you). The means the aliens use to Mind Control on some of your techs to get them to sabotage your base defenses is stated to be a one-off.
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In Marathon 2, Durandal gets frustrated by this trope. He has a clever plan to introduce a virus into Pfhor war machines and turn them against their masters. It works... for about twenty minutes, before the Pfhor render it ineffective. Turns out another alien race called the Nar had that same idea twenty years ago, and the Pfhor have set up countermeasures against such tactics.
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In chapter three of The Adventures of Dr. McNinja, Dan McNinja defeats Frans Rayner by punching him in the right butt cheek and exposing his neckbeard. Years later, when Dr. McNinja fights him, Frans has moved his weak spot to a different location and taken extreme precautions to keep his beard from ever being noticeable.
Also applies to holy weapons throughout the series: it's one banishment per weapon, and if the numbers suddenly change on you, you'd better have another trick up your sleeve.
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In the original game, NOD only had the capability to launch a single nuke (it was a lot more powerful than nukes in the later C&C games though). GDI's counterpart, the Ion Cannon had no such limitations.
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Supergirl (2015): Shortly after Kara arrived on Earth, she used her powers to save someone from a car accident. Her foster father Jeremiah covered by claiming she got lucky, but warns her that excuse is only going to work once. It's almost fifteen years before she finally becomes a hero, starting when she has to save her sister's crashing plane. As it turns out, the explanation didn't even work perfectly that one time. Rick Malverne, a friend Kara's age, was always suspicious after that incident and quickly put two and two together when Supergirl saved a plane with Kara's sister on it.
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In Wicked, Elphaba's control over her magic is limited, often gives unexpected results, and she can't undo a spell once it's been cast. This leads to problems throughout the story.
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The Treasure Juju in Homestuck can only ever be used twice, but each use is so drastically different that they could technically be different items. The first time, it can only be used by Caliborn as a weapon against his enemies (by sealing them away inside it forever). The second time, it can only be used by Caliborn's enemies against him (by unleashing their power in one concentrated blast).
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In the Counter Monkey episode "The Bardic Knock Spell", Spoony discusses this in relation to RPGs, saying that if you can impress the Game Master, he'll let you get away with doing something really audacious, but he'll only give it to you once. The example he gives, the titular "spell", involves gaining access to the villains' stronghold by just knocking on the front door, then punching out whomever answers.
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In Tales of Symphonia: Dawn of the New World, Emil's Mystic Arte Ain Soph Aur can be used on Richter the first time, but if you try using it again, he will counter it with a Mystic Arte of his own, magnifying its damage output and targeting your entire party.
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Ultimate Marvel
Ultimate Human included Tony Stark curing Bruce Banner of the Incredible Hulk. The catch was that if the Hulk returned even once, its physiology could adapt and the cure would never work again. (See Doomsday, below) Predictably, by the end of the comic they are placed in a situation that requires the return of the Hulk.
All-New Ultimates: There's a giant in town, but Kitty can not repeat the trick she used against Galactus. She needed helpt from SHIELD to be turned into a giant, and there's no SHIELD anymore.
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Star Trek: Insurrection features a perfectly justified and logical case. The Sona's subspace weapons create a subspace tear that threatens to destroy the Enterprise. Riker and Geordi conclude that ejecting the warp core and detonating it in the tear will seal it. It works perfectly, but as Geordi points out they don't have another warp core, and the Sona can always do it again. Fortunately, Riker thinks of a way to keep them from doing so.
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The console-only game (later ported to mobiles) Civilization Revolution only has one nuke in any single game. It's given to the player who builds the Manhattan Project wonder. It can hit any city or square on the map but leaves no fallout. Doesn't stop enemies from declaring war on you at the drop of a hat.
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The Order of the Stick: Vaarsuvius tells Durkon that V's brief period of unimaginable magical power was "a singular event" that can't be repeated and doesn't disagree when Durkon asks if it had a heavy Cast from Experience Points cost. It's true that it almost certainly can't be repeated... because the power came from a Deal with the Devil that the Fiends almost never grant, and comes with a debt that Vaarsuvius hasn't even started to pay off.
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Mentioned in Mud. Mud was bitten by a snake as a child and had his life saved by having an antidote administered in time. Unfortunately since the antidote is made of horse blood, it's something the body will recognize as a foreign material after being administered once and reject...meaning if bitten again there will be no way to save him and he will die.
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In the first episode of the series, a hectic encounter with hostile aliens forces Space Dandy to reveal a decoration on the Aloha Oe that is actually an explosive powerful enough to an destroy an entire planet. If he had though it through, he would've realized that detonating it spells doom for everybody, including himself and his crew.
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In Persona 5, this applies to the Phantom Thieves, who force a change of heart onto a person by stealing the root of their distorted desires, their "Treasure", from that person's Mental World. To make the Treasure take physical form so that they can steal it, the Thieves have to send their target a Calling Card in the real world. The shock and paranoia from the warning are what make the Treasure manifest, but those emotions won't last long and the Thieves can't repeat the process; they only get one day to steal the Treasure.
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The final boss of Undertale's Neutral Route empowers himself by absorbing the six human souls the monsters had collected by that point. As the battle progresses, you call for aid and the souls answer, rebelling against the one using them and eventually enabling you to win. This character also possesses a Ripple Effect-Proof Memory of your gameplay, so on subsequent playthroughs, if you reach the Neutral route's final boss again he'll opt not to go through with it since he knows the souls will simply revolt against him again and the end result will be the same.
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John Dread from Max Steel pulls this one on Max. The first time around when Max tricked Dread into punching his claw into magma it was quickly melted off and he was defeated. The second time it happens, Dread lampshades that he fell for the same trick twice, but this time around his claw is made of an alloy that won't melt at that temperature. Cue Oh, Crap! face from Max as he's now facing Dread with a red hot glowing claw.
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Superman: The Animated Series saw the Man of Steel use this trope frequently. When his foes escaped from Cardboard Prison, they'd usually find Superman wearing a suit designed to be proof against their powers. Somewhat subverted, though, in that these suits were not foolproof and the villains found ways around them (or just ripped them off).
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Schlock Mercenary had a Time Travel storyline; but the particular method could only work once due to some of the equipment being destroyed in the process (and before the effective start of the conditions that made it useable in that way), meaning only one shot...
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In Wizards of Waverly Place, Alex defeats Franken Girl, the monster Justin created to guard his bedroom, after finding out that Justin installed his own laptop as brain and tricking the monster into shutting itself down with the "Control", "Alt" and "Delete" keys. Unfortunately for Alex, Justin brings Franken Girl back to life and adds a spell lock preventing Alex from using the same trick.
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Space Adventure Cobra: Cobra's arch enemy of the Three Sister's Arc, Crystal Boy, is Nigh Invulnerable to just about every attack Cobra used against him. Near the end of the Arc Cobra faces off against him and after a long fight he catches him off guard by launching his fake arm at him via the Psychogun, presumably killing him. When he appears in the Psychogun OVA Cobra attempts to kill him the exact same way only to have him catch the fist mid air and proclaim that the same trick wasn't going to work twice.
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Silent Hill 3: The Seal of Metatron from the first game. Granted, Alessa knew how to use it (having been Dahlia's daughter and all), and she only sought to contain "God" and the Otherworld within her area. Alessa's next incarnation though she may be, Heather is ignorant on how it works; and it is reportedly "difficult to control," indeed making it "just a piece of junk"...in her hands. Presumably Vincent doesn't know either, seeing as he would've told her how to use it otherwise. Leonard was only appointed its guardian, expecting a member of "the order" to be the chosen one to use it, so it's also doubtful that he knew how to use it. Claudia may or may not have known the required ritual, but denies its efficacy. Oh, well...at least Harry left Heather a little present.
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Something similar happens in Batman Beyond. Inque was vulnerable to freezing and water in her first appearance, but in later appearances learned to curl herself into a ball, protecting the bulk of her mass from being frozen and breaking free in short order, or modifying her chemical makeup to resist water. Batman himself uses this against Inque, protecting himself from being enveloped by her with an electricity based contact defenses.
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Most of a PC's powers in 4th Edition Dungeons & Dragons only work once per encounter or once per day. At least they can be recharged between fights, though...
For arcane or divine spells, this is justified by encounter and daily spells requiring a minute or two or longer of memorization, preparation, and/or prayer to prepare. For physical powers, it's explained that they are so physically demanding or reliant on surprise tactics that your character can only perform (or get away with them) once. This is one of the main reasons why there's a Broken Base over this edition: some players find that it's not airtight, but in most cases it works under Rule of Cool; other players find this a ridiculous case of Fridge Logic.
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Pacific Rim: This is actually the main reason Gypsy Danger's Armsword is used as a secret weapon. The Kaiju are all connected, with information one wave learns during their attacks sent back to the Kaiju leaders while designing the next waves. This allows them to analyze the earth tech and lets them come up with ways to neutralize them. It is this, combined with the increased number of attacks, that puts humanity in the bind that it is in by the movie's start.
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Before the release of Fallout 4, an excited fan sent Bethesda a box of real-life bottle caps weighing over 11 pounds with a tongue-in-cheek letter asking Bethesda if this would be an acceptable substitute for payment. To his surprise, Bethesda then sent him an email accepting the "payment", and agreeing to send him a copy with the understanding that it'll only work the one time.
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In Chuubo's Marvelous Wish-Granting Engine, which has plenty of powers that are very draining if you use them more than once in a chapter or even once in a book, and a few (such as some forms of Reality Syndrome) that simply cannot be used more than once per chapter, the Salvation power from the Wounded Angel arc works once per campaign. It is the only power in the entire corebook with this limitation. To be fair, it's a strong power, enabling you to reshape the world to save someone for whom all is lost, but still!
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In one of the Legion of Super-Heroes try-out issues, one of the would-be Legionnaires was a woman called X-Bomb Betty, who had the power to "create an explosion of 150 million megatons". When they asked her to demonstrate this she got a panicked look and said "But ... But I can only do it once!"
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This is how all of the Adepts' spells work in Anthony's Apprentice Adept series, with the same variation loophole.
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In Batman: Arkham City, each of the takedowns against Mr. Freeze only work once, as he'll adapt to them. You must find five unique methods of taking him down. In the higher difficulty modes, you have to figure out even more ways to attack him.
In the comic book tie-in Batman comes up with the idea of disguising the Bat-boat as a fishing boat to sneak into Arkham City. It works brilliantly...until he tries it a second time, where the boat winds up blown up with a rocket. Lampshaded by Batman.
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According to Things Mr. Welch Is No Longer Allowed to Do in an RPG, sending a stripper to the guard office follows this principle.
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In the SMG4's Mario Bloopers episode "Mario and the T-Pose Virus", the T-Pose Virus caused by Waluigi is undone by using 1-up Mushrooms. In "The Mario Convention", Waluigi creates another T-Pose Mushroom, which turns Meggy (and others) into T-Pose zombies. Mario tries using a 1-up Mushroom on Meggy, only for it to have no effect due to Waluigi's Rejection powers being stronger this time around.
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In Resident Evil: Outbreak: File #2, you find an Explosive Leash control for the rampaging tyrant, which you can use to skip one fight with him. It doesn't kill him, though, and if you use it early he will return.
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The Bartolls of the Super Robot Wars Original Generation OVA series worked on this trope - should an attack kill one of them, they would instantly adapt to it and find ways to dodge it or create ways to protect themselves. By the end of the second episode, Cybuster's forced to use CyFlash and the SRX is forced to use the Hyper Tromium Cannon just to wipe out a small army of them.
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Spidey once came across Stilt-Man wreaking havoc. He was disappointed, as the villain had designed a mask to keep Daredevil from hitting him in the face with his stick. Spidey just webs him to a lamppost.
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This is a matter of some contention among the The Lord of the Rings fandom, among whom there is a vocal minority that considers Aragorn's use of a seemingly invincible undead army in only one battle (rather than throughout the war) to be a Headscratcher verging on a Wall Banger. Debates about whether the Exact Words of their oath would have allowed him to demand longer service from them can (and have!) gone on for dozens of pages in various internet fora. The popular consensus is that the undead army was not magically compelled to obey Aragorn, but did so voluntarily as he was the only one who could release them to pass on to a more standard afterlife. If they'd gotten it into their heads that he did not intend to release them, they could very well have gone over to the other side, so he decided that it was better to be safe than sorry. YMMV on whether this explanation makes this a legit example or is merely a convenient bit of Fan Wank. It should also be noted that it is never made clear (in the books at least) if the army of the dead can physically hurt people, as the only army they deal with they simply terrify into fleeing. If they could not actually attack people, once the psychological impact of them wore off, their usefulness would be greatly reduced. Regardless, they demanded to be released after a single battle, and he complied.
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In Fantastic Four, many of Reed Richards' attempts to cure The Thing have been of the only-works-once variety. These always come with a reversal method which Ben will inevitably use, sacrificing his humanity to stop the villain.
When Kristoff Vernard was uploaded with Doctor Doom's memories to take his place after his apparent death, he decided to attack the FF by reusing an old plan to send the Baxter Building into orbit, reasoning that it had only failed the first time the true Doom attempted it because of the treachery of Namor; the plan failed again, but only because Kristoff's uploaded memories didn't include the Invisible Girl's ability to generate forcefields, allowing the FF to survive the building exploding in space and return to Earth.
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We Happy Few: When a person remembers the past and refuses to take their Joy for long enough, they become a "downer"; in other words, they're a perfectly normal person in a world gone insane. Joy no long has quite the same intoxicating effects for them as it once did. It wears off very quickly, and leaves the individual extremely depressed, hungry and thirsty.
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In the final episode of The Big O Roger Smith finally puts some of the pieces of the Myth Arc together and unlocks the Wave-Motion Gun built into his mecha just in time to put an end to the Big Bad. Wouldn't you know: He missed, and firing the one shot burns the gun to a crisp.
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In Gojira, the Oxygen Destroyer is a one-shot weapon used to kill Godzilla, and its creator ensures that it can never be reproduced by destroying his notes and killing himself. In Godzilla vs. Destoroyah, the device turns out to have mutated dormant Precambrian organisms into another Kaiju, leading the humans to come up with an elaborate plan to lure Godzilla into a fight with it.
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In Final Fantasy VII: Advent Children Complete, Cloud Strife uses Omnislash in an attempt to finish off Sephiroth the same way as he did in the original Final Fantasy VII. Unfortunately for him, it backfires spectacularly, because Sephiroth is more than ready this time. Instead, Sephiroth's the one who makes a repeat performance by skewering Cloud through the stomach like he did during their skirmish in Nibelheim years before when Cloud goes in for the final slash. Sephiroth even takes the time to mock him for it, before showing his wing and stabbing Cloud multiple times in mid-air with Octaslash.
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In comics, the monster Doomsday (who once killed Superman) had "evolution" powers, such that he could adapt to counter attacks once used against him — i.e., having once been beaten by a Green Lantern's ring, he became relatively immune to its power. In Superman/Doomsday: Hunter/Prey, the Radiant's energy attack which killed Doomsday in the past no longer has any effect on the creature when the recreated Radiant tries it again on him.
Superman's revival after his death at the hands of Doomsday is considered this because of the circumstances behind it, involving a certain revitalization chamber and, possibly, Jonathan Kent having a near-death experience with him.
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Paper Mario 64: To some degree, this happens in the first (non-hopeless) fight with Bowser; his invincibility can be canceled out with the Star Beam you've spent the entire game earning. In the second a short time later, the move is now useless and you must get one better to win.
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Final Fantasy XV has a retroactive version as of the release of Episode Ardyn. Two years before Noctis was born, Ardyn and Ifrit attacked Insomnia; after the playable happenings, Ifrit is watching as Ardyn tries to strike down King Regis, and gets impaled on Bahamut's Ultima Sword when the Bladekeeper interrupts. Thirty-two years later, when Noctis comes to Insomnia for the Final Bosses, he fights Ifrit again; this is the only time when Noctis is able to summon Bahamut to perform Ultima Sword, and Ifrit dodges every one.
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Champions. The villainous robot Mechanon has automated factories around the world. Whenever he's destroyed, one of the factories creates him again, this time with defenses against whatever destroyed him. This makes it much or difficult or even impossible to destroy him the same way again.
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Gems of War: One-Shot * spells, which can only be used once per battle.
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Marionettes: The first time Trixie faces Gear Shift and Cover Story, she covers the floor with ice to make them slip. In their second encounter, when she tries it again, they're wearing winter horseshoes that allow them to walk on ice.
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It Only Works Once
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In an issue of Uncanny X-Men Rogue absorbed Nightcrawler's powers and defeated Nimrod, the mutant killing robot from the future, by teleporting a chunk of his body away. The next time the X-Men fought Nimrod, Nightcrawler simply tried the same trick again, only to discover that Nimrod had adapted himself so that not only did it not work but the attempt messed Nightcrawler up quite badly.
Similarly, Sentinel robots in general had the ability to learn from experience (and transmit this information to other Sentinels), so a trick used on a Sentinel wouldn't work again on any Sentinel. Their ability to physically adapt to is more limited than Nimrod, so anything that beats a Sentinel through brute force will usually still work.
In Marvel's Heroes Reborn arc Doctor Doom had guard robots that had this programmed into them.
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When Superboy was dying after a particularly strenuous fight Robin was able to find a cure in one of Lex Luthor's secret labs. However the cure could only be used once and any attempt to use the same concoction again after having used it once would kill Conner.
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The Kim Possible episode "Queen Bebe" and The Powerpuff Girls episode "The Boys Are Back In Town" both have the heroines confronting a previously-defeated opponent and trying what they used last time, only to find that it doesn't work anymore.
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Technical Machines (TMs) were initially one-use items and often limited to one per-game, so you only had one opportunity to teach Blizzard to a Pokémon. Pokémon Black and White onwards, however, made them have infinite uses.
Some TMs were buyable in the first four generations, so the player technically could teach certain TM moves to multiple members of their party. However, the TMs could still only be used once apiece, so players would have to repeatedly buy them to do so.
Generation VIII mixes up the formula; TMs remain infinite in usage, but the moves that they teach are often on the weaker side. TRs (Technical Records), on the other hand, are single-use variants that generally teach much stronger moves.
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Rosario Vampire: Brightest Darkness Act III: While the group is fighting Kuyou in chapter 41, Mizore and Kurumu manage to land a blow on him with their duet. In chapter 42, when they try the attack again, Kuyou isn't about to let it happen twice and disrupts the attack by throwing Moka at them.
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The Spectacular Spider-Man: In the Villain Team-Up episode, Doc Ock explicitly points out that none of the methods Spider-Man used to stop his foes last time will work: Doc Ock's arms are powered by Electro, the power pack on Vulture's back is better armored, Electro has much better control of his power (such that he can safely contact water), Shocker and Rhino know better than to confront him in an enclosed space, and um...there aren't any concrete mixers around to trap Sand-Man. It goes both ways, though, since Spidey's also been upgraded since the last time he fought them.
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Negi's Pactio in Mahou Sensei Negima! allowed use of all his partner's abilities and Asuna's antimagic (to an extent) but was merely used as a tool to keep Rakan from using his own Pactio. And after that, it was canceled. Obviously for the sake of drama (such a game breaker power) but seriously, you would think something useful like that would be kept around. Although he could easily regain it by making a Pactio with someone else.
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Back to the Future Part III: Mad Dog has a gun hidden in his hat, but it's so small that it can only carry a single bullet, and it's foiled when Marty uses a Frisbee Pie Pan to deflect his one and only shot.
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On an episode of The Adventures of Jimmy Neutron: Boy Genius, the title character builds nanobots in a flying saucer to defend him from bullies. They malfunction and begin to threaten everyone who comes within 5 feet of him. He defeats them Logic Bomb style by beating himself up. Take 2: Jimmy re-hires the Nanobots as Beta Readers for his homework assignments, and they take their "correcting errors" to extremes, going so far as to start correcting human error (bad posture, fashion, etc.) He tries to Logic Bomb them again by saying that flaws indicate that humans are functioning perfectly, but they stop trying to puzzle it out, call it "extreme error" and just start deleting people off the face of the earth. Somewhat subverted, though, in that he still uses their programming to overload them; he purposefully miscalculates a number on his homework, and asks the nanobots to correct it. The number in question? Pi, which no computer could calculate to perfection.
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In Dragon Ball Super, Trunks and Mai attempt to use this this skill on Zamasu, unable to beat the immortal god by himself or with Goku and Vegeta's power. However, because they forgot the seals to prevent escape, Zamasu's able to escape and flips the hell out.
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A Gumby cartoon had Gumby returning a wand to a magician who left it behind at Gumby's lemonade stand (it made sense then). The magician rewards Gumby with a wand that will perform a single trick. Gumby uses it to repair his mother's pitcher that got broken at the start of the cartoon.
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In Tensou Sentai Goseiger, Robogog's ultimate weapon was a world ending Wave-Motion Gun called Calamity Destruction, but it could be used once, and thanks to Buredoran, who intentionally didn't seal Gosei Knight's morpher completely, Gosei Knight was able to block the blast and stop it from destroying the world, leaving Robogog furious that his ultimate weapon had been wasted.
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In the Star Trek: The Original Series episode "Let That Be Your Last Battlefield", Kirk, Spock, and Scotty initially manage to get Bele to give control of the Enterprise back by activating the ship's Self-Destruct Mechanism, which he complies with before the sequence is complete. Towards the end of the episode, after hijacking the ship again, Bele manages to burn out the mechanism, preventing them from using it again.
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Command & Conquer series:
In the original game, NOD only had the capability to launch a single nuke (it was a lot more powerful than nukes in the later C&C games though). GDI's counterpart, the Ion Cannon had no such limitations.
In the Yuri's Revenge expansion of Red Alert 2, partway through the fourth Allied mission in Egypt you get one free use of the Psychic Dominator after you rescue Einstein from Yuri's clutches - he managed to sabotage it under Yuri's nose before your forces rescue him. He states it is unstable, though, and will self-destruct before you get a second use.
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Spider-Man:
This ended up happening by the eighth or so issue of The Amazing Spider-Man. In the Vulture's first appearance, Spider-Man took him down by simply clipping his wings with a device designed to cancel out the magnetic harness that allowed him to fly. Not so in their second encounter, where the Vulture puts in a fail-safe to get around this, and sucker-punches Spidey the minute he isn't looking.
Similarly, after being blinded with a squirt of webbing, Doc Ock started treating his glasses with a non-stick coating.
Spidey once came across Stilt-Man wreaking havoc. He was disappointed, as the villain had designed a mask to keep Daredevil from hitting him in the face with his stick. Spidey just webs him to a lamppost.
In an old Marvel Team-Up issue, Black Panther concocted a super strong web fluid to hold Stegron, a villain that had previously broken out of Spider-Man's webs. It was stated that the new serum was very unstable, and Black Panther himself warned Spider-Man that it could only be used on this one occasion.
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The Good Place:
After Eleanor works out that "The Good Place" is actually a psychological Hell for her and three other humans, she thwarts Michael's plan to reset their memories by writing herself a note and hiding it in Janet's mouth. While this allows her to uncover the truth much quicker without her old memories, Michael simply reboots their memories again, and checks Janet's mouth as he restarts the neighbourhood.
Partway through Season 2, Jason prevents demons from capturing him and his friends using a quick Molotov Cocktail and a Battlecry. Next season, he tries the same thing to defeat another group of demons - only for one demon to clamp a hand over Jason's mouth while another douses the Molotov.
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Jack Sparrow's pistol in Pirates of the Caribbean: The Curse of the Black Pearl. When he was marooned, his former crew gave him a gun with one bullet to kill himself with. Indeed, he only fires the gun once in the film.
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One Piece:
Numerous villains seem to just give up after an ass-kicking by Luffy and co. despite the fact that they could logically resume their activities after the Straw Hats have left the area. Especially since Luffy and company nearly never actually kill the Big Bad. Most of the time, they go a ways to justify why the Big Bad doesn't do this, but occasionally they don't bother.
First example: Captain Kuro. Kuro had planned to leave a life of piracy by instilling himself as a butler of a rich child. After a few years, he was going to have his hypnotist force her to write Kuro into her will, then kill her and take her money. Instead, he gets beat up and forced back out to sea. Though he couldn't have pulled this trick again on the same island (and would need to adjust the plan for a lack of a hypnotist), no one else knew he was alive and he could have tried elsewhere. Yet he's still a pirate as far as we know. Later, it's revealed that the Marines learned that Kuro had faked his death; this is likely because the hypnotist had joined the Navy after Kuro's defeat, but it's never made clear.
Next is Buggy, who had taken over a town. Luffy beats him and sends him flying. He could always just stay the course. However, Buggy being Buggy, he holds a grudge and starts following Luffy instead. This also happened to Alvida, who joined Buggy.
Crocodile looks this way since his loss of Warlord of the Sea status means he can no longer operate out in the open without drawing attention from the Marines, but his criminal organization still exists. A miniarc revealed that after Luffy defeated him, Crocodile lost interest in the outside world, which is why he doesn't bother escaping jail with most of the rest of his Officer Agents. However, when he heard about the impending war with Whitebeard and the World Government and Luffy showed up on Level 6 attempting to rescue his brother, he offered his power to help them escape, his interest rekindled by a chance to strike back at the pirate who ruined his dreams of becoming King. And with that pirate's death, he's ready to give it another shot; the next time Crocodile is seen after that arc, he has plans to head towards the New World again.
On the topic of Alabasta, there's Nami's Clima-Tact; Usopp had made it little more than a tool for parlor tricks, but the Tornado Tempo was specifically described as an attack that can work only once. This is due to the attack relying on wound springs inside one of the 3 poles. Once it fires it looks like it would take significant effort to reload (think trying to re-coil a stretched out slinky) and many of Nami's attacks require all 3 poles so losing one means her fight is basically over regardless of if she's won or not.
Brook's Devil Fruit grants him the ability to come back from the dead... once. Since his soul took too long to find his body, he is now a living, still killable skeleton. But with training, he has subverted the trope by expanding on his power, making its ability to revive him only one aspect of his powers.
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Some particularly gutsy BattleBots competitors have chosen to equip their bots with weapons that can only be used once per match:
Chrome Fly has an otherwise fairly standard design, but it has a companion drone that drops a cylindrical weight. As the drone cannot retrieve this weight and can only carry one at a time, it can only use it once. As luck would have it, the one time it dropped the weight in its entire career, it missed.
Double Jeopardy has a cannon as its main weapon that shoots a similarly shaped, sized weight, though at about 190 miles per hour (or about 305 kilometers per hour). Like Chrome Fly's drone, it can only shoot it once, as it's not equipped to reload another slug. Unlike Chrome Fly, it managed to hit its opponent Gamma 9 on its initial match, though Gamma 9's operators were never directly in front of Double Jeopardy whenever possible, which meant it didn't fire that cannon until right before it was knocked out.
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Pokémon:
The Master Ball, which has a 100% chance of capturing any Pokémon, but you're only guaranteed a single one in each game, which becomes especially frustrating in later generations as the number of stupidly difficult-to-catch Pokémon increases. You can get more through the lottery in most games, but the default odds are 1 in 100,000.
There is an item called the Sacred Ash, which revives all fainted Pokémon in your party AND fully restores their HP. The catch is that it appears almost exclusively in the presence of wild Ho-Oh, so there's only one in the game. The Festival Plaza in Sun & Moon, however, lets you purchase it.
Technical Machines (TMs) were initially one-use items and often limited to one per-game, so you only had one opportunity to teach Blizzard to a Pokémon. Pokémon Black and White onwards, however, made them have infinite uses.
Some TMs were buyable in the first four generations, so the player technically could teach certain TM moves to multiple members of their party. However, the TMs could still only be used once apiece, so players would have to repeatedly buy them to do so.
Generation VIII mixes up the formula; TMs remain infinite in usage, but the moves that they teach are often on the weaker side. TRs (Technical Records), on the other hand, are single-use variants that generally teach much stronger moves.
There are a few moves like Overheat and Draco Meteor that, while very powerful, cut the user's Special Attack stat in half. Naturally, this means that it should wipe out whatever it attacks because the Pokémon will be effectively useless for the remainder of its time out of the ball. This can be remedied by recalling it, but as that's the obvious move, the opponent will be able to predict you doing that.
A more extreme version would be sacrificial moves which cause the user to faint. These range from support (such as the stat-lowering Memento and the healing Lunar Dance) to attacks (such as the high-power Explosion or the damage-equal-to-lost-HP Final Gambit).
The move Fake Out is a high priority attack that makes the target flinch 100% of the time, but it only works on the first turn the user is sent out.
The same goes for First Impression, a Bug-Type variant more than twice as powerful that was introduced in Generation VII.
Z-Moves are devastatingly powerful and always accurate, but can only be used once per battle. Not per Pokémon, but for the whole battle.
Mega Evolution and Dynamaxing are the same as Z-Moves: extremely powerful, but only one Pokémon can use it per battle. And while Mega Evolution lasts until the user faints, Dynamaxing only lasts for three turns.
Burn Up requires the user to be a Fire-type to use, and causes them to stop being Fire-type when used.
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Godzilla:
In Gojira, the Oxygen Destroyer is a one-shot weapon used to kill Godzilla, and its creator ensures that it can never be reproduced by destroying his notes and killing himself. In Godzilla vs. Destoroyah, the device turns out to have mutated dormant Precambrian organisms into another Kaiju, leading the humans to come up with an elaborate plan to lure Godzilla into a fight with it.
In the first of the films featuring Mechagodzilla, Godzilla was able to defeat his Evil Knockoff by tearing off his head, removing the controls and cameras that let the mechanical monster keep moving. The second time they faced off, Godzilla repeated the move and was shocked when a second, smaller head emerged from the gap and blasted him with lasers.
In Godzilla (1998), the military first manage to lure Godzilla out of hiding by using a huge pile of fish as bait. After they bungle this attempt at killing Godzilla, they try again, but place the bait in the middle of Central Park this time, where Godzilla will be completely exposed. Unfortunately for them, Godzilla doesn't fall for it twice and stops short before turning back and running away.
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Doctor Strange once was turned into a vampire by Dracula, who boasted that since Dracula rules over all vampires, Strange was unable to attack him. Indeed, try as he might, Strange could no longer call upon his usual sources for spells: Aggamotto, Cyttorak, Watoomb, etc. In desperation, and with effort that nearly killed him, Strange called out the name of God.
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In Man of Steel, the first time Superman and Zod fight, Zod's helmet gets broken and subjects him to Sensory Overload. By the time they have their rematch, Zod has learned to focus his senses.
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Lelouch's mind control power in Code Geass only works once on any specific person. The duration can be permanent if worded correctly, but if the effect ends, that individual can never again be affected by the power. That is, unless Jeremiah uses his Geass Canceler to undo the effects. But even then, if he does, Lelouch's power can work on someone again.
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Fate/Grand Order: In her original life, Queen Medb from Celtic Mythology was assassinated by having a hunk of cheese hurled at her with a slingshot while she was bathing. During the Summer 2017 event, the heroes attempt to have Enkidu hurl a hunk of cheese at her while she is taking a shower. She deflects it and says she's developed a Spider-Sense for cheese.
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In The Bible, Satan's Eden ploy was this. Yes, it struck an incredible blow against humanity, but it also exposed Satan as a deceiver who is Always Chaotic Evil. This is a big reason why Christianity's tone is so triumphant: there is nothing Satan can do to us that will be as bad as what he's already done, and even that didn't erase our intrinsic worth in the eyes of God. Notably, the next time Satan tried to tempt a representative of humanity, he was shut down hard.
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In the original Metal Gear Solid, feel free to hide in a cardboard box right in front of the enemy. If they see you, they'll look at the box in confusion, then move on. However, if the same enemy sees that same box in the same place, he'll pick it up. Apparently, it's more suspicious for the box to still be there than for it to suddenly disappear.
In Metal Gear Solid 3: Snake Eater, you can use the mask given to you at the start of your mission during Colonel Volgin's first bossfight to stall him, making him believe you're his lover, Ivan Raikov. Whether you choose to get in a free hit or just wait too long, he will eventually wise up and aggressively charge at Snake, never being fooled by that ploy again.
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In Kuroko no Basuke, this is the critical drawback of Misdirection Overflow. After its effect wears off, not only will that technique not work anymore on the team it was used on, but that team will also become immune to the most basic of misdirection techniques. It's described in-universe as "performing a trick while simultaneously showing how it's done."
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In Mega Man X3, you have the ability to swap out with Zero. It can only be used once per stage and if he dies, you lose him for the rest of the game. He's also needed to get his saber near the end of the game.
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Leprechauns in the Fablehaven series cannot be caught by the same trap twice. Patton Burgess not only caught one five times with five different traps, he had more traps ready for anyone who wanted to try it.
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Bryan Danielson ended his best of five series against Homicide to get a shot at the Ring of Honor World Champion with a 100 rotation air plane spin. When he tried to use it again during the title match, Austin Aries slipped out of it after 30 rotations. Danielson's inability to take the title from Aries led to him going into voluntary exile from ROH until he knew he could do it.note CM Punk ended up beating Aries during this time, but Danielson's time off still paid off with a World Title reign of his own.
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The Kairosect from Trollhunters is an artifact that allows its user to step outside of time, and while it actually only works three times, Blinky wastes the first two uses demonstrating it.
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Teen Titans:
In "Divide and Conquer", when Cinderblock puts Starfire in a bearhug, she knocks him back by kicking him in the face. In "Aftershock Part 1", this scenario happens again, but this time, he blocks her kick.
In "Titans East Part 2", Cyborg is the only Titan left who can resist Brother Blood's Mind Control. Blood has effortlessly torn Cyborg's limbs off, paralyzed the other Titans, and is ripping Cyborg's circuitry apart, trying to find the component that makes Cyborg immune. Cyborg announces "It's my SPIRIT!", instantly rebuilds himself, ignores Blood's energy blasts (the ones that blew his limbs off 15 seconds ago) and takes Blood out with one punch. They at least lampshade it this time.
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Granblue Fantasy: Quite a few characters have skills that can only be used once per battle. Most notably, each one of the ten Eternal characters has an incredibly powerfull skill with an extremely long 10 turn cooldown before it can be used. In addition, some other characters have skills that grant them massive buffs but knock themselves out after a few turns, meaning anything you do with them until then will only have one chance to work in each battle.
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In Wing Commander: Prophecy, the protagonists capture an alien plasma weapon capable of destroying a fleet with one shot, install it to their carrier and successfully use it to destroy an enemy blockade. They then determine it would be too dangerous to try recharging it for a second shot.
Humorously described by one of the characters as "Fire and Forget. We fired it once, now we can forget about ever using it again."
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Ender's Game has a strategy winning a training battle that is discussed to Only Work Once (it is technically an exploit of the battleroom rules). It works. A similar strategy comes into play in the end battle, recalling that moment.
Ender is constantly challenged by the teachers, who keep putting him in situations that prevent the use of old tactics, necessitating him to come up with new ones on the fly. Not only that, but the other armies keep adopting his tactics, so he can't use the same ones anyway.
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JoJo's Bizarre Adventure: Straits/Straizo in Part 2 had the powerful Space Ripper Stingy Eye, an ability he witnessed in Part 1 where a vampire can shoot fluids from the eyes at a ridiculously high velocity, allowing it to cut through flesh. The first time he fires, he misses a fatal shot at Joseph, but nicks his neck. The next time he tries it, he hits a mirror reflection of Joseph, and then the third time, it is completely reflected. Joseph lampshades that he only needed to see the attack once to know how to counter it, due to the predictability that Straits would go for the fatal shots in the neck and forehead again.
Subverted in Part 1 where Jonathan is caught off guard by Dio's attack of the same kind, as the first time it was used, he was plummeting off a cliff and didn't see it firsthand. The second time it is used, he had no idea it was coming.
The above two can also be grouped together, as the attack ended up being fatal to Jonathan in the first real encounter with it, but the story of the attack was passed down through Erina to Joseph, her grandson. As a result, it never succeeds from the start on Joseph because he already knew how the move worked.
Part 3's Anubis inverts this trope. Along with its unique traits, its skill in swordsmanship is so extreme that using the same attack twice against it is completely ineffective.
Later on in Part 7, during the climax of the D4C arc, Gyro uses the Super Spin Technique to attack Valentine. This attack rapidly ages cells to the point of death. Though technically successful, an imperfection in the steel ball cancels the perfect rotation, allowing Valentine to survive the attack. Gyro doesn't get another shot. Johnny later attempts the same feat, but Valentine's warning prevents the Dio from another world from suffering the same fate.
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Fate/EXTRA CCC: Karna has access to one of the most powerful Noble Phantasms seen in the franchise: Vasavi Shakti, the spear granted to him by the god Indra. The spear has sufficient power to quite literally kill a god, and its raw destructive power far exceeds nearly all others. However, unlike most Noble Phantasms, Karna can only ever use this ability once, and he has to permanently give up his Armor of Invincibility to do it.
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In Gundam Build Fighters, the Star Build Strike's Absorb Shield wasn't designed this way, but that tends to be how it works out in practice. As the name implies, the shield opens up and absorbs any beam attacks that come its way, then uses the energy to power some rather impressive Limit Breaks; after its spectacular first use, most of the smarter fighters (and this being the World Championship, that meant most of them) realize what's going on and go out of their way to disable the shield first or attack with solid projectiles like missiles that can't be absorbed.
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Fairy Tail
Averted with the Iced Shell spell, which works in the flashback with Gray's teacher Ur sacrificing herself to seal away and eventually kill the demon Deloria. Every subsequent attempt to use Iced Shell in the present fails not because it wouldn't work, but because Natsu is there to stop Gray from trying to throw away his life needlessly when they can beat the enemy the old-fashioned way without dying.
Natsu states his secret weapon against Zeref is this. Because it's the last of Igneel's power, he has no way to regenerate more of it once it's used up. He can still unleash a facsimile in "Fire Dragon King" mode, but it's not as potent as Igneel's power was.
In Fairy Tail: 100 Years Quest, Erza states that the weakness of hypnotism-based magic is that once a person knows how the specific spell works on the target, they can counter further usages of it, going so far as to call said weakness "understanding." She proves it by No Selling Kyria's "cutting mentality" spell in their rematch after being subjected to and recovering from its effects from their first fight.
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Fate/stay night: A problem when facing Servant Berserker. He's incredibly powerful, so to manage to damage him at all is quite an effort... but he has the God Hand Noble Phantasm, which allows him to revive himself 11 times, so you'll have to kill him twelve times to finally finish him off. Worse, he becomes immune to whatever it was that killed him last time, so you have to do it twelve different ways. Of course, there's nothing saying a sufficiently powerful attack can't take away more than one of his lives at once.
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In Krull the seer known as the Widow Of The Web can protect vision seekers trying to enter her domain, in the center of a giant spider web, by turning over a large hourglass which immobilizes the giant spider guardian while the sands fall. We learn that leaving is impossible, because "it can be turned only once. That is the lure of the web."
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In Metal Gear Solid 3: Snake Eater, you can use the mask given to you at the start of your mission during Colonel Volgin's first bossfight to stall him, making him believe you're his lover, Ivan Raikov. Whether you choose to get in a free hit or just wait too long, he will eventually wise up and aggressively charge at Snake, never being fooled by that ploy again.
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SpongeBob SquarePants
One episode has a very picky customer invoke an Achilles in His Tent by making SpongeBob doubt his skills as a fry cook by claiming he forgot the pickles. When SpongeBob returns and confronts him again, he pulls the same trick, but SpongeBob quickly realizes how he got away with it when he starts laughing.
Another episode has a giant clam snatch Mr. Krabs' millionth dollar, with Mr. Krabs sobbing until SpongeBob and Squidward decide to help him get it back. After trying to fool him with a different dollar, Mr. Krabs starts sobbing again, only for Squidward to say that it won't work again (Mr. Krabs decides to resort to more drastic measures instead).
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The Kido Cannon in Bleach: Memories of Nobody burns itself to ash after being fired only once.
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Pokémon:
Any Pokémon with the move Overheat, though most notably Ash's Torkoal, could only use it once at full power. This has its roots in the games, where, while the move can be used multiple times, it cuts the Special Attack by half whenever it is used.
Ash's Snivy's Attract only works once for an opponent. After that, they find a way to break through it.
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The Beyonders: If Maldor sends out a torivor to duel, it's all but guaranteed to kill whoever he sent it after. However, once the torivor has killed its mark, it is freed from the bindings on it and can return to its native realm. Since Maldor has neither the power nor the knowledge to summon more torivors, he usually sticks to sending them out to spy.
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In the first season finale of Witchblade, Sara chooses to go back in time to the first episode, thus undoing everything that happened in the series so far. She is specifically informed that this will only be possible to do once, presumably to stop that ability from being a Game-Breaker.
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Suicide Squad: Hell to Pay features a 'Get out of Hell' card as the main plot point, hinted to be the only one in existence and sought after by multiple dying and concerned about death parties to bypass punishment in death. It ends up being used up by Bronze Tiger when Deadshot leaves it on his dying body, though Amanda Waller is initially unaware of this when Deadshot gives it to her, she finds out in the tie-in comic that she had been duped. The comic focuses on her attempt at finding the second best thing available, a Key that also bypasses eternal damnation but doesn't necessarily sends someone to Heaven like the Card, and its also even harder to find.
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My Little Pony: Friendship Is Magic:
The Elements of Harmony against Discord. This time they're dangling the elements over his head as leverage to force him to play along while they try and reform him, but the first two things Discord does is destroy all of Twilight Sparkle's "reform spells" and sweet-talk Fluttershy into promising to never use her element against him, rendering the rest completely useless.
Word of God is that this is why Celestia never used the Elements in the show. She and Luna originally used them to seal Discord, but then Luna turned into Nightmare Moon. Even Celestia wasn't powerful enough to use the Elements on her own, so she had to use a spell to be able to wield them by herself against Nightmare Moon—but the cost was that she'd never be able to use the Elements again.
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In Sheep, Dog 'n' Wolf, Ralph Wolf uses a magic flute to hypnotize Sam Sheepdog and lure him away from the flock of sheep. It works so well, it's available to use in the next level. However, it's revealed that it won't work anymore as Sam now has earplugs.
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Doctor Who:
"Bad Wolf"/"The Parting of the Ways":
Jack jury-rigs a BFG from the Defabricator and his Compact Laser Deluxe. However, it only has enough power to kill one Dalek, due to the makeshift nature of the weapon. When it returns in "The Stolen Earth"/"Journey's End", Jack's had the time to upgrade and fine-tune it so it no longer dies after one shot.
Rose absorbs the Time Vortex, effectively making her a God, allowing her to reduce the Daleks to dust with a wave of her hand. However, the side effect is that it very nearly kills her, and causes the Doctor to regenerate after he absorbed the energy from her.
The power the Doctor gains over the Carrionites in "The Shakespeare Code" when he uses the species' name to banish one of them only works once. Oddly, though, it only works once AT ALL, rather than only working once on each member of the species.
"Resolution": It's implied the reason the villain evades the Doctor's attempt to roast it inside its own casing with repurposed microwave parts is because this is basically the same way it was first defeated on Earth in the ninth century.
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In the Yuri's Revenge expansion of Red Alert 2, partway through the fourth Allied mission in Egypt you get one free use of the Psychic Dominator after you rescue Einstein from Yuri's clutches - he managed to sabotage it under Yuri's nose before your forces rescue him. He states it is unstable, though, and will self-destruct before you get a second use.
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In UFO, the invading aliens use a new superweapon in each attack. It fails not necessarily because it is flawed but also through bad luck. However, they never try it again although it still exists and might be devastating in another attempt.
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In Tremors, each trick used to defeat a graboid will not work on the next one, because the creatures are too smart and adapt just too damn fast. For example, they kill one by tricking it to swallow a pipe-bomb, but when they try on a second one, it spits the explosive back at them. The trend continues to reappear throughout the sequels.
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In Quest for Glory IV, the ultimate joke is this. Anyone who hears it will laugh, no matter how little a sense of humor they have, but only the first time. You use it to distract the Big Bad long enough for you to kill him.
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The Mafuba/Evil Containment Wave in Dragon Ball was able to seal King Piccolo away in a rice cooker in his backstory, but fails all three times it's used on him or his son/reincarnation:
Roshi tries to use it on King Piccolo, but it fails simply because he missed the jar.
Tien also planned to use it against King Piccolo, but in the manga practicing the technique broke the jar before he could even try. In the anime he gets a chance to try it, but Drum jumps in front of the wave himself and King Piccolo destroys the jar before Tien can even seal him.
Kami tries to use it on Piccolo (so he can stop him without killing him and thus himself), but Piccolo just turns it on him, mentally gloating, "If you thought this incarnation would be beaten like the last, then you have not thought!"
In Dragon Ball Super, Trunks and Mai attempt to use this this skill on Zamasu, unable to beat the immortal god by himself or with Goku and Vegeta's power. However, because they forgot the seals to prevent escape, Zamasu's able to escape and flips the hell out.
Likewise, it's revealed at the beginning of Dragon Ball Z that Shenron, and the Earth's Dragon Balls by extension, cannot grant the same wish twice; for example, if a person who has been already been resurrected is killed again, the person in question will be Killed Off for Real. Porunga, and the Namekian Dragon Balls by extension, have no such limitation.
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In the first season of Workaholics, the guys got rehired after threatening to expose their boss' illegal business practices. This is never, ever brought up again, even in the numerous following episodes where she bullies them or makes their lives difficult in some way.
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After Optimus Primal's death in the first season finale of Beast Wars, the first few episodes of the second season see Rhinox manage to bring Primal back to life by using a recently-created rift into transwarp space and a blank stasis pod (essentially a Transformer body without its own soul) to bring Optimus back to life. The show itself explicitly states that the rift Rhinox is using will close soon and it was by relative chance that the Maximals found the blank pod when they did, and even then Rhinox experiences considerable strain and puts his own life at risk trying to retrieve Optimus's spark before the rift closes.
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Nasuverse:
Fate/stay night: A problem when facing Servant Berserker. He's incredibly powerful, so to manage to damage him at all is quite an effort... but he has the God Hand Noble Phantasm, which allows him to revive himself 11 times, so you'll have to kill him twelve times to finally finish him off. Worse, he becomes immune to whatever it was that killed him last time, so you have to do it twelve different ways. Of course, there's nothing saying a sufficiently powerful attack can't take away more than one of his lives at once.
Fate/EXTRA CCC: Karna has access to one of the most powerful Noble Phantasms seen in the franchise: Vasavi Shakti, the spear granted to him by the god Indra. The spear has sufficient power to quite literally kill a god, and its raw destructive power far exceeds nearly all others. However, unlike most Noble Phantasms, Karna can only ever use this ability once, and he has to permanently give up his Armor of Invincibility to do it.
Fate/Grand Order: In her original life, Queen Medb from Celtic Mythology was assassinated by having a hunk of cheese hurled at her with a slingshot while she was bathing. During the Summer 2017 event, the heroes attempt to have Enkidu hurl a hunk of cheese at her while she is taking a shower. She deflects it and says she's developed a Spider-Sense for cheese.
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In an episode of Andromeda, the Nietzscheans are trying to erase the AIs of captured High Guard warships. They have finally created a device that can do that without any physical damage, and manage to delete the AI of the Wrath of Achilles. Dylan captures the device and keeps it in storage. A year later, a rogue AI named Gabriel hijacks a newly-built sister-ship of the Wrath of Achilles (Siege Perilous class), and Dylan uses the device again. However, Gabriel has been aware of the device's existence for this whole year ("For an AI, it's an eternity") and has managed to devise a countermeasure. To be fair, though, it would still probably work on any AI not aware of the device's existence.
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Downplayed in Amazing Fantasy. Izuku can only use his Venom Strike once a day, limiting him to one usage of it in any given fight, but he'll be able to use it again after a full night's rest.
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 It Only Works Once / int_d23e6c9a
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It Only Works Once / int_d23e6c9a
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It Only Works Once
 It Only Works Once / int_d56cb367
comment
Power Rangers S.P.D.: Kat Manx is given a special Morpher that allows her to transform, but only has one charge, and it only lasts an hour of transformation time. She takes it and goes into battle as the Kat Ranger for the first and only time.
Subverted with her Tokusou Sentai Dekaranger counterpart Swan Shiratori, who can use her DekaSwan form whenever she wants, but makes a policy of only doing so once every four years (except in emergencies, as when she tried to transform in the finale only to be interrupted before she could do so). She transforms again in Kaizoku Sentai Gokaiger.
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 It Only Works Once / int_d616724d
type
It Only Works Once
 It Only Works Once / int_d616724d
comment
In League of Legends, Poppy's ultimate ability, diplomatic immunity can turn out like this. Offensively, she uses it to take down an enemy champ without fear of repercussion from the rest (Unless they crowd control her and force her to waste it.) but once they're gone, she is immediately vulnerable and they'll likely get her down so she doesn't assassinate the rest of the team. However; it's fully possible for her to ult someone else, then wreak havoc without touching them, or declare Diplomatic Immunity in someone else and uses it to escape, which doesn't work just once....
Or any of the ap nukes, notably Katarina and leblanc, once you use up your whole nuking combo... you can only do small non-damaging auto-attacks. Good thing their cd are short, and katarina have her passive... This is why ap loses their powers late game.
A better example is the concept of the carry in general which can become this when taken to extremes. If one person on the enemy team noticeably gets lots of kills, the most common counter tactic is to wait for the opportunity for all 5 members of your team to dogpile that champion since they're clearly the biggest threat and need to be eliminated on sight. If they don't properly account for this, then their domination of the early game becomes their one shot, and the rest of the game is it not working since their enemies never let them act freely again.
 It Only Works Once / int_d616724d
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1.0
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It Only Works Once / int_d616724d
 It Only Works Once / int_db6a22a6
type
It Only Works Once
 It Only Works Once / int_db6a22a6
comment
Biggles found himself fighting Sky Pirates targeting bullion shipments in Biggles and Co. The first time they hit his aircraft, he thwarted them with a false floor in the aircraft and some decoy crates full of lead... with some explosives wired to go off when the lid was lifted. The second time it happened, the air pirates immediately went for the secret compartment... And got another load of lead ingots because he'd put the real gold in the main cargo hold. The third time, the now very annoyed air pirates discovered they'd forced down an unloaded decoy aircraft. That didn't work quite so well because the air pirates had an inside source who'd hidden a smoke bomb in the latest consignment.
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 It Only Works Once / int_dd814a79
type
It Only Works Once
 It Only Works Once / int_dd814a79
comment
REDLINE: The president of Roboworld uses the Three-Point Disintegration Cannon to try to kill Funky Boy. Unfortunately, Funky Boy regenerates, while the Cannon needs two hours to recharge.
 It Only Works Once / int_dd814a79
featureApplicability
1.0
 It Only Works Once / int_dd814a79
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 REDLINE
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It Only Works Once / int_dd814a79
 It Only Works Once / int_de8f6b17
type
It Only Works Once
 It Only Works Once / int_de8f6b17
comment
In Godzilla (1998), the military first manage to lure Godzilla out of hiding by using a huge pile of fish as bait. After they bungle this attempt at killing Godzilla, they try again, but place the bait in the middle of Central Park this time, where Godzilla will be completely exposed. Unfortunately for them, Godzilla doesn't fall for it twice and stops short before turning back and running away.
 It Only Works Once / int_de8f6b17
featureApplicability
1.0
 It Only Works Once / int_de8f6b17
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 Godzilla (1998)
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It Only Works Once / int_de8f6b17
 It Only Works Once / int_dff248f8
type
It Only Works Once
 It Only Works Once / int_dff248f8
comment
In The Adventures of Puss in Boots, this is how all magical fountains work. You can only benefit from the effects of a fountain once, and if you say "release" to negate the effects, you don't get a second chance.
 It Only Works Once / int_dff248f8
featureApplicability
1.0
 It Only Works Once / int_dff248f8
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 The Adventures of Puss in Boots
hasFeature
It Only Works Once / int_dff248f8
 It Only Works Once / int_e1d125d7
type
It Only Works Once
 It Only Works Once / int_e1d125d7
comment
The GeoGrey Sword in Digimon Savers; after its first appearance, it ceases to work on anyone, up to and including the villain in The Movie.
In Digimon Xros Wars there's the digimemories which have the data of legendary Digimon, and can summon them to use their abilities. But they can only be used once for each zone.
 It Only Works Once / int_e1d125d7
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1.0
 It Only Works Once / int_e1d125d7
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It Only Works Once / int_e1d125d7
 It Only Works Once / int_e3349241
type
It Only Works Once
 It Only Works Once / int_e3349241
comment
The reason why Diabolik and inspector Ginko don't bother reusing plans and gadgets: the next time the police will have figured out how to counter Diabolik's tricks, and Diabolik will know how to get around the counter, as shown by Diabolik's perfect masks (one of the rare tricks he does reuse: the police checks faces multiple times if they even suspect he's around, and sometimes Diabolik can pass through the checks if the circumstances are right). However this is valid only if the opponent knows what happened, as otherwise they can't figure a way out of it.
 It Only Works Once / int_e3349241
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1.0
 It Only Works Once / int_e3349241
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It Only Works Once / int_e3349241
 It Only Works Once / int_e5c5bc22
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It Only Works Once
 It Only Works Once / int_e5c5bc22
comment
The GURPS Player Handbook suggests the DM should reward a clever player who comes up with a new gambit by letting the trick work ... but only once. The example given is throwing sand in an opponent's eyes to blind him — might work once, but if it were really that effective all the time, "fighters would leave their weapons at home and carry bags of sand instead." Thus to keep the game balance, creative gambits should only work once.
 It Only Works Once / int_e5c5bc22
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 It Only Works Once / int_e694aadb
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It Only Works Once
 It Only Works Once / int_e694aadb
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In the ninth Ciaphas Cain book, an Imperium astropath discovers how to project a psychic field that jams the Tyranid Hive Mind, turning the tide of a major battle. Unfortunately, the strain burned out the captured Tyranid cortical node that was generating the jamming signal, and it will be very hard to find another one so nearly intact.
 It Only Works Once / int_e694aadb
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 It Only Works Once / int_e71c679
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It Only Works Once
 It Only Works Once / int_e71c679
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Subverted with her Tokusou Sentai Dekaranger counterpart Swan Shiratori, who can use her DekaSwan form whenever she wants, but makes a policy of only doing so once every four years (except in emergencies, as when she tried to transform in the finale only to be interrupted before she could do so). She transforms again in Kaizoku Sentai Gokaiger.
 It Only Works Once / int_e71c679
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It Only Works Once / int_e71c679
 It Only Works Once / int_e7a9c06c
type
It Only Works Once
 It Only Works Once / int_e7a9c06c
comment
In Paperinik New Adventures' fourteenth issue, a shadow creature can only be destroyed by a weapon created specifically for the job; the heroes, however, didn't have enough time to manufacture more than two charges for the weapon. As Paperinik puts it: "Be careful where you aim, you won't get a third shot."
 It Only Works Once / int_e7a9c06c
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 It Only Works Once / int_e7a9c06c
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It Only Works Once / int_e7a9c06c
 It Only Works Once / int_ea4f62db
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It Only Works Once
 It Only Works Once / int_ea4f62db
comment
Subverted in Family Guy. Peter and Brian get rid of James Woods by leading him along with pieces of candy and trapping him in a crate. When he comes back later, he steals Peter's identity and they go through a long ordeal, but then Peter and Brian eventually get rid of him in the exact same way with Peter pointing out that they should have tried this again from the beginning.
 It Only Works Once / int_ea4f62db
featureApplicability
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 It Only Works Once / int_ea4f62db
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 Family Guy
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It Only Works Once / int_ea4f62db
 It Only Works Once / int_ef076a36
type
It Only Works Once
 It Only Works Once / int_ef076a36
comment
In the first level of the second game (based on the last episode of Star Trek: Voyager), the Borg adapt to the I-Mod as well by using a jamming field specifically designed to stop the I-Mod from firing. The player is forced to do the mission with regular weapons, using shots sparingly.
 It Only Works Once / int_ef076a36
featureApplicability
1.0
 It Only Works Once / int_ef076a36
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 Star Trek: Voyager
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It Only Works Once / int_ef076a36
 It Only Works Once / int_efaeff60
type
It Only Works Once
 It Only Works Once / int_efaeff60
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In Titan Maximum, Palmer believes that his favorite move, the Sucker Punch works every time. However his brother Billy, points out that attack can only work once in each fight because it is quite impossible to catch the guy off guard again.
 It Only Works Once / int_efaeff60
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 It Only Works Once / int_efaeff60
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It Only Works Once / int_efaeff60
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It Only Works Once
 It Only Works Once / int_f2d501d6
comment
This trope is used as an actual story element in Runaways. Nico's weapon, the Staff of One, can cast any spell whatsoever but then can never cast it again. (Attempting to repeat a spell results in random effects.) It essentially serves as a leash to her otherwise godlike Green Lantern Ring powers. However, it turns out that she can only use each specific spell with a specific code word once; in a team-up with the Young Avengers, she was able to cast the same spell repeatedly by running through The Vision's language banks, while on another occasion she was able to 'reuse' a 'Freeze' spell by instructing her enemies to freeze at a specific temperature.
 It Only Works Once / int_f2d501d6
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It Only Works Once / int_f2d501d6
 It Only Works Once / int_f2da188a
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It Only Works Once
 It Only Works Once / int_f2da188a
comment
Both used and subverted in The Batman episode "Meltdown". Batman first defeats Clayface by throwing a freeze-bomb at him. When he tries it later, Clayface said he saw it coming and just made his body hard so it bounced off. Then Detective Yin does this after pretending she was going to shoot him which prompted the line:
 It Only Works Once / int_f2da188a
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It Only Works Once
 It Only Works Once / int_f42fa008
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Hotel Transylvania: The monsters of Transylvania can only "zing" once in their whole life.
Subverted in the third film however.
 It Only Works Once / int_f42fa008
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 It Only Works Once / int_f42fa008
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It Only Works Once
 It Only Works Once / int_f4559fd1
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During Kinnikuman's fight against Atlantis of the Seven Devil Choujin, the ghosts of the previously killed Devil Choujin interfere, forcing the Justice Choujin to use a magic glass to become spirits and stop the interference. When Sunshine and Ashuraman use a similar trick during their match against the Machine Guns during the Dream Tag Tournament, the other Justice Choujin try to use the glass again, only to be rebuffed by the Mountain, who knew they were coming.
 It Only Works Once / int_f4559fd1
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 It Only Works Once / int_f4559fd1
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It Only Works Once / int_f4559fd1
 It Only Works Once / int_f4daf1b9
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It Only Works Once
 It Only Works Once / int_f4daf1b9
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Squirtle in Ashes of the Past decided to one up Gary's Blastoise and his Mega Evolution and created Squirtlite, a Mega Stone for Squirtles. That's right, Squirtle created his own Mega Evolution. However, upon reverting back to normal the Squirtilite explodes in a massive burst of water. Averted as Squirtle can simply create another one until he figures out how to stop the Squirtlite from destroying itself after use.
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It Only Works Once / int_f4daf1b9
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It Only Works Once
 It Only Works Once / int_fc5b5922
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Brought up in Species II with Sil/Eve's alien race. If they survive being exposed to a disease, after healing, they immediately develop an immunity to it, leaving it ineffective later on.
 It Only Works Once / int_fc5b5922
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 It Only Works Once / int_fc5b5922
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 It Only Works Once / int_fd933142
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It Only Works Once
 It Only Works Once / int_fd933142
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In Tomica Hero Rescue Force the team combine all their vehicles into Great God Striker and use it's Super Final Rescue to destroy the Big Bad Batsu. Unfortunately it fails.
 It Only Works Once / int_fd933142
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 It Only Works Once / int_fd933142
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It Only Works Once / int_fd933142
 It Only Works Once / int_fdbace96
type
It Only Works Once
 It Only Works Once / int_fdbace96
comment
The Quantum Destabilizer gun from Gravity Falls zig-zags this trope. Dipper and Ford try to use this against Bill Cipher in Weirdmageddon Part 1. Ford directly acknowledges that they only have one shot, and while the shot does damage Bill, it fails to kill him. Ford is captured, and the weapon is never able to be used again.
 It Only Works Once / int_fdbace96
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 It Only Works Once / int_fdbace96
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It Only Works Once
 It Only Works Once / int_fdd79060
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In Marvel's Heroes Reborn arc Doctor Doom had guard robots that had this programmed into them.
 It Only Works Once / int_fdd79060
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 It Only Works Once / int_fdd79060
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It Only Works Once / int_fdd79060
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It Only Works Once
 It Only Works Once / int_fe83990a
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In a similar instance to the Saint Seiya example above, an OVA pitted (female) Ranma and Akane versus two powerful sisters (never happened in the manga). After getting creamed in their first match, the two leads train for a rematch. The sisters' ordinary attacks then prove ineffective against Akane (who has trained up to their level) and their special skills are useless against Ranma (who explicitly says, "Your wind attack ain't gonna get me twice! I've seen it once and it's easier to avoid it!")
 It Only Works Once / int_fe83990a
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It Only Works Once
 It Only Works Once / int_ff9ab17f
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In Star Trek: The Next Generation, the Borg have the ability to adapt to phaser frequencies after a few shots. The obvious workaround for this is changing the phaser frequency to something they haven't adapted to. The Star Trek: Elite Force games explicitly addressed this issue by introducing the Infinity Modulator (or I-Mod) gun, which changes frequencies too quickly for Borg to adapt.
In the first level of the second game (based on the last episode of Star Trek: Voyager), the Borg adapt to the I-Mod as well by using a jamming field specifically designed to stop the I-Mod from firing. The player is forced to do the mission with regular weapons, using shots sparingly.
The I-Mod actually infinitely randomizes the frequency to such an extent that the Borg can't even adapt to the randomization algorithm normally used by phasers.
In the series itself, much is made of this trope when anyone is simply planning on fighting the Borg. They know that any possible advantage they can come up with will result in the Borg adapting to render it ineffective quickly afterward. Thus, they often hold back the advantage until it is most likely to be needed.
 It Only Works Once / int_ff9ab17f
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 It Only Works Once / int_ff9ab17f
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The following is a list of statements referring to the current page from other pages.

 It Only Works Once
processingCategory2
Speculative Fiction Tropes
 Digimon Savers / int_20e6a6e7
type
It Only Works Once
 Fate/Grand Order - Absolute Demonic Front: Babylonia / int_20e6a6e7
type
It Only Works Once
 Magical Girl Lyrical Nanoha A's / int_20e6a6e7
type
It Only Works Once
 Panty & Stocking with Garterbelt / int_20e6a6e7
type
It Only Works Once
 The Big O / int_20e6a6e7
type
It Only Works Once
 Astro City (Comic Book) / int_20e6a6e7
type
It Only Works Once
 Avengers Academy (Comic Book) / int_20e6a6e7
type
It Only Works Once
 Doctor Octopus (Comic Book) / int_20e6a6e7
type
It Only Works Once
 Lady Shiva (Comic Book) / int_20e6a6e7
type
It Only Works Once
 Nico Minoru (Comic Book) / int_20e6a6e7
type
It Only Works Once
 Paperinik New Adventures (Comic Book) / int_20e6a6e7
type
It Only Works Once
 Secret Wars (2015) (Comic Book) / int_20e6a6e7
type
It Only Works Once
 Superman: The Doomsday Wars (Comic Book) / int_20e6a6e7
type
It Only Works Once
 Teenage Mutant Ninja Turtles (IDW) (Comic Book) / int_20e6a6e7
type
It Only Works Once
 The Black Knight (Comic Book) / int_20e6a6e7
type
It Only Works Once
 The Death of Superman (Comic Book) / int_20e6a6e7
type
It Only Works Once
 Ancienverse / Fan Fic / int_20e6a6e7
type
It Only Works Once
 Fractured / Fan Fic
seeAlso
It Only Works Once
 Intrepid / Fan Fic / int_20e6a6e7
type
It Only Works Once
 A Tale of Transmigration (Fanfic)
seeAlso
It Only Works Once
 Chibipoes Acceleration (Fanfic)
seeAlso
It Only Works Once
 PokemonMastersOfAzeroth
seeAlso
It Only Works Once
 Retry Reload Respawn (Fanfic) / int_20e6a6e7
type
It Only Works Once
 Son of the Sannin (Fanfic) / int_20e6a6e7
type
It Only Works Once
 The Legend of Total Drama Island (Fanfic) / int_20e6a6e7
type
It Only Works Once
 With This Ring (Fanfic) / int_20e6a6e7
type
It Only Works Once
 Yugioh EQG (Fanfic) / int_20e6a6e7
type
It Only Works Once
 Home Alone / int_20e6a6e7
type
It Only Works Once
 Iron Man 2 / int_20e6a6e7
type
It Only Works Once
 It's a Mad, Mad, Mad, Mad World / int_20e6a6e7
type
It Only Works Once
 Kiss of the Dragon / int_20e6a6e7
type
It Only Works Once
 Liar Liar / int_20e6a6e7
type
It Only Works Once
 Man of Steel / int_20e6a6e7
type
It Only Works Once
 Power Rangers (2017) / int_20e6a6e7
type
It Only Works Once
 Return of the Jedi / int_20e6a6e7
type
It Only Works Once
 Shin Godzilla / int_20e6a6e7
type
It Only Works Once
 The Amazing Spider-Man 2 / int_20e6a6e7
type
It Only Works Once
 The Great Muppet Caper / int_20e6a6e7
type
It Only Works Once
 The Mighty Ducks / int_20e6a6e7
type
It Only Works Once
 Achievement Hunter Minecraft Series (Lets Play) / int_20e6a6e7
type
It Only Works Once
 Ascendance of a Bookworm / int_20e6a6e7
type
It Only Works Once
 Black Bullet / int_20e6a6e7
type
It Only Works Once
 Bruce Coville's Book of... / int_20e6a6e7
type
It Only Works Once
 Ciaphas Cain / int_20e6a6e7
type
It Only Works Once
 Fine Structure / int_20e6a6e7
type
It Only Works Once
 Lacuna / int_20e6a6e7
type
It Only Works Once
 The Beyonders / int_20e6a6e7
type
It Only Works Once
 The Genesis Fleet / int_20e6a6e7
type
It Only Works Once
 The Magicians / int_20e6a6e7
type
It Only Works Once
 The Maximus Universe / int_20e6a6e7
type
It Only Works Once
 The Three Investigators / int_20e6a6e7
type
It Only Works Once
 AdditionalEvilOverlordListCellblockD
seeAlso
It Only Works Once
 British Royal Family
seeAlso
It Only Works Once
 MacGuffin Girl
seeAlso
It Only Works Once
 Detective Conan (Manga) / int_20e6a6e7
type
It Only Works Once
 Get Backers (Manga) / int_20e6a6e7
type
It Only Works Once
 Goldfish Warning! (Manga) / int_20e6a6e7
type
It Only Works Once
 Inuyasha (Manga) / int_20e6a6e7
type
It Only Works Once
 Ushio and Tora (Manga) / int_20e6a6e7
type
It Only Works Once
 Yamada-kun and the Seven Witches (Manga) / int_20e6a6e7
type
It Only Works Once
 Fringe / int_20e6a6e7
type
It Only Works Once
 Hikari Sentai Maskman / int_20e6a6e7
type
It Only Works Once
 Kamen Rider 555 / int_20e6a6e7
type
It Only Works Once
 Kamen Rider Kuuga / int_20e6a6e7
type
It Only Works Once
 Land of the Lost (1974) / int_20e6a6e7
type
It Only Works Once
 Level Up / int_20e6a6e7
type
It Only Works Once
 M*A*S*H / int_20e6a6e7
type
It Only Works Once
 Robot Wars / int_20e6a6e7
type
It Only Works Once
 Stranger Things / int_20e6a6e7
type
It Only Works Once
 The Pretender / int_20e6a6e7
type
It Only Works Once
 V (1983) / int_20e6a6e7
type
It Only Works Once
 War of the Worlds (1988) / int_20e6a6e7
type
It Only Works Once
 Witchblade / int_20e6a6e7
type
It Only Works Once
 Arkham Horror: The Card Game (Tabletop Game) / int_20e6a6e7
type
It Only Works Once
 Mordheim (Tabletop Game) / int_20e6a6e7
type
It Only Works Once
 Ace Combat 7: Skies Unknown (Video Game) / int_20e6a6e7
type
It Only Works Once
 Arknights (Video Game) / int_20e6a6e7
type
It Only Works Once
 Chrono Trigger (Video Game) / int_20e6a6e7
type
It Only Works Once
 Death Road to Canada (Video Game) / int_20e6a6e7
type
It Only Works Once
 Gothic (Video Game) / int_20e6a6e7
type
It Only Works Once
 Kingdom Hearts: Chain of Memories (Video Game) / int_20e6a6e7
type
It Only Works Once
 Leisure Suit Larry (Video Game) / int_20e6a6e7
type
It Only Works Once
 MS Saga: A New Dawn (Video Game) / int_20e6a6e7
type
It Only Works Once
 Marathon (Video Game) / int_20e6a6e7
type
It Only Works Once
 Mega Man Maker (Video Game) / int_20e6a6e7
type
It Only Works Once
 Metal Gear Solid 2: Sons of Liberty (Video Game) / int_20e6a6e7
type
It Only Works Once
 Path of Exile (Video Game) / int_20e6a6e7
type
It Only Works Once
 Plants vs. Zombies: Heroes (Video Game) / int_20e6a6e7
type
It Only Works Once
 Puzzle & Dragons (Video Game) / int_20e6a6e7
type
It Only Works Once
 Quest for Glory (Video Game) / int_20e6a6e7
type
It Only Works Once
 Silent Hill 3 (Video Game) / int_20e6a6e7
type
It Only Works Once
 The Jackbox Party Pack (Video Game) / int_20e6a6e7
type
It Only Works Once
 The Last Story (Video Game) / int_20e6a6e7
type
It Only Works Once
 The World Ends with You (Video Game) / int_20e6a6e7
type
It Only Works Once
 XCOM 2 (Video Game) / int_20e6a6e7
type
It Only Works Once
 XCOM: Enemy Unknown (Video Game) / int_20e6a6e7
type
It Only Works Once
 Yandere Simulator (Video Game) / int_20e6a6e7
type
It Only Works Once
 Outlast / Videogame / int_20e6a6e7
type
It Only Works Once
 Mahou Tsukai no Yoru (Visual Novel) / int_20e6a6e7
type
It Only Works Once
 Phoenix Wright: Ace Attorney – Spirit of Justice (Visual Novel) / int_20e6a6e7
type
It Only Works Once
 asdfmovie (Web Animation) / int_20e6a6e7
type
It Only Works Once
 White Dark Life / Web Comic / int_20e6a6e7
type
It Only Works Once
 Critical Role (Web Video) / int_20e6a6e7
type
It Only Works Once
 Archie Sonic Online (Webcomic) / int_20e6a6e7
type
It Only Works Once
 The Dementia Of Magic (Webcomic)
seeAlso
It Only Works Once
 Overused Sci-Fi Plot Devices (Website) / int_20e6a6e7
type
It Only Works Once
 Amphibia / int_20e6a6e7
type
It Only Works Once
 Avengers, Assemble! / int_20e6a6e7
type
It Only Works Once
 Beast Wars / int_20e6a6e7
type
It Only Works Once
 Jackie Chan Adventures / int_20e6a6e7
type
It Only Works Once
 Rick and Morty / int_20e6a6e7
type
It Only Works Once
 Star Wars: The Clone Wars (Season 1 — Season 6) / int_20e6a6e7
type
It Only Works Once
 Teen Titans Go! To the Movies / int_20e6a6e7
type
It Only Works Once
 The Incredibles / int_20e6a6e7
type
It Only Works Once
 The Spectacular Spider-Man / int_20e6a6e7
type
It Only Works Once
 The Swan Princess / int_20e6a6e7
type
It Only Works Once
 Trollhunters / int_20e6a6e7
type
It Only Works Once
 Winx Club / int_20e6a6e7
type
It Only Works Once
 Young Justice - Original Series / int_20e6a6e7
type
It Only Works Once
 Bryan Danielson (Wrestling) / int_20e6a6e7
type
It Only Works Once
 Kazuchika Okada (Wrestling) / int_20e6a6e7
type
It Only Works Once
 Digimon Xros Wars / int_20e6a6e7
type
It Only Works Once
 Mawaru-Penguindrum / int_20e6a6e7
type
It Only Works Once
 All-New Ghost Rider (Comic Book) / int_20e6a6e7
type
It Only Works Once
 Facing the Future Series (Fanfic) / int_20e6a6e7
type
It Only Works Once
 itonlyworksonce
sameAs
It Only Works Once
 Wizards of Waverly Place / int_20e6a6e7
type
It Only Works Once
 BlazBlue: Chrono Phantasma (Video Game) / int_20e6a6e7
type
It Only Works Once
 Iron Man: Armored Adventures / int_20e6a6e7
type
It Only Works Once