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Room 101

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A Place Worse Than Death. The place no one wants to visit: a torture chamber, brainwashing program or place of punishment so terrible that it is spoken of only in whispers. No one knows just what is done there; those who are sent there either never return, or else are so traumatized that they cannot speak of the torments they experienced there.
The Trope Namer is the room in the Ministry of Love from Nineteen Eighty-Four, where it is eventually revealed that those who are sent there are made to literally live out their worst nightmares. No matter what spark of individuality you cling to — hope, love, loyalty, whatever it is — you WILL be made to betray it in Room 101. You then irrevocably cross the Despair Event Horizon. As one character says, "You really do mean it, and nothing is ever the same afterward."
Many that take after the Nineteen Eighty-Four example also tend to mimic the seemingly mundane name of the room, being structured to just a location and a number. The way it's treated and responded to by characters is what gives it its foreboding and terrifying aura.
When the threat is used humorously, it can sometimes be known as the Maximum Fun Chamber, or involve Cool and Unusual Punishment and Suckiness Is Painful. Often, the audience has to Take Our Word for It that You Do Not Want To Know what happens within. May also involve Animorphism. The widespread presence of these places in an Evil Empire may or may not be a sign of Industrialized Evil, depending on how personalized they are.
For the metaphysical equivalent see Cosmic Horror Story. The effect is the same, there's a... thing so terrible that it is spoken of only in whispers. No one knows just what it is; those who seek it either never return, or else are so traumatized (or, more likely, are driven so completely insane) that they cannot speak of the horrors they experienced by encountering it.
The name of the Nineteen Eighty-Four room inspired a TV series of the same name, in which celebrities sent their pet hates (including, in one controversial case, the Welsh) to Room 101.
Can be a Sealed Room in the Middle of Nowhere. Can also overlap with Place Worse Than Death. No relationship to Subject 101. Or Room 222, for that matter — unless the Ministry of Love knows the single episode of a soap opera can drive you nuts. Related to Psychological Torment Zone (a Location 101, if you will) and Black Bug Room (the Mental World equivalent). Sometimes employed for The Ludovico Technique.
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The HQ of the Cable Street Particulars in Night Watch, one of the series' darkest moments. There is a chair for people to be strapped into, there is a gully on the floor for blood to run, the walls are heavily soundproofed, and there is a single tooth left on the floor.
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Matilda: Mrs. Trunchbull sends misbehaving pupils to the Chokey, a small piece whose walls are adorned with nails and broken glass.
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20th Century Boys: People who resist being brainwashed at Friend Land (which is already bad enough) are sent for more advanced treatment to Friend World, which is never actually described.
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Inside Job (2021): 'Shadow Prison X' is described as a mixture of this, Hellhole Prison, and The Alcatraz, stated to be a 'place so secret even [Cognito] doesn't know where it is' and filled with torture devices.
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 Inside Job (2021)
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Dark Angel: Manticore's Reindoctrination process for rogue or misbehaving genetic experiments. It's partly revealed to include excruciating brainwashing sessions via laser beam through the eye, and other forms of physical and psychological torture.
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Arrow: In Season 5, Oliver Queen is injected with a hallucinogen that will make him experience his worst fears, then locked in a cell with a pistol loaded with one bullet.
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The red bag in Ideal. To put it in perspective, gangster Psycho Paul is said to have 'cried like a little girl' after looking inside it, and even Ax-Crazy hitman Cartoonhead is scared of it.
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Mother Base in Metal Gear Solid V: The Phantom Pain has a room used for interrogations referred to as "Room 101". In addition to being an obvious reference to Nineteen Eighty-Four, it also refers to its location on the base; level 1, Warehouse 01 of the Command Platform.
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In Shades of Grey, people whose merit score dips down low enough get sent to Reboot, where they are supposedly retrained to fit into society. It turns out to just be an 'abandoned' town with a town hall painted with a color that kills you.
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In Hollow Fields, underachieving students are sent to detention in "The Windmill". No one who goes in ever comes out again because the teachers are using the children sent there as test subjects for a Grand Theft Me experiment. They sort of... break... in the process.
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In The Shining, Room 217 (Room 237 in the Kubrick version) serves the same basic purpose. Danny Torrance initially surmises that certain areas of the Overlook Hotel are just as dangerous as Room 217 and should be avoided.
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Mahoraba has Shirogane's own Room 101 inside her classroom for students who misbehave in class or failed to do homework. When not in her classroom, she might borrow some random room for dealing punishments to her students. Played for laughs, misbehaving students are just heard screaming by other students and comes out pale as a ghost with no one ever willing to say what really goes on behind those doors.
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 Mahoraba (Manga)
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The Simpsons: In "Burns' Heir", a man is told that Bart has been brainwashed by Mr. Burns and has to Deprogram him. He tries to do this in the room 101 of a motel.
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Urinetown in Urinetown has this reputation. It turns out, though, that there is no Urinetown; those who are sent there are just thrown off a building to their deaths.
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Sid Meier's Alpha Centauri:
The Punishment Sphere facility. Drones — the uneducated, disaffected unskilled worker class — will never, ever riot if you have one of these. It's never explained what's done (though according to the sound file that plays when you build it, one man who was tough enough to take it quickly broke when his wife was put in...), but whatever goes inside must be really, really, really nasty, because building it also halves that city's scientific research output and prevents citizens from becoming Talents, the educated and creative class, which also prevents that base from ever entering an intellectual Golden Age. When a rival faction is defeated by the player, a short clip (one for male, female, and Progenitor) shows that leader in a Punishment Sphere.
The game also references "nerve stapling" as the dictatorial method of quashing Drone Riots. Nerve stapling is implied to lobotomize its victims into mindless workers. Not necessarily gruesome per se, but certainly a flagrant violation of human rights nonetheless (and a horrific punishment by any measure).
In fact, using the Nerve Staple option to "calm" your populace is considered an Atrocity by the other factions, and will temporarily do horrible, horrible things to your economy as everyone stops trading with you for several turns (which can be devastating for any faction that relies on trade routes and external income, as in the other Civ games) and you get temporarily chucked off the Council.
If you manage to get the UN Charter repealed by the Council, then it is no longer considered an atrocity to nerve-staple people or use nerve gas on civilians. Planet busters are still a no-no.
In Dragon Sun by Michael Ely, nerve stapling is developed by Yang's scientists in order to more effectively control his population and create a nigh-unstoppable army. When the others find out about it, they are utterly horrified and put Yang in jail for life. The victims they find have some sort of electrodes in their heads used to induce intense pain.
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Room 9 from season 2 of Heroes. Maury's apartment where Matt Parkman and Nathan Petrelli are trapped within Maury's illusions of a twisted alternate of the S1 "Bomb" Future.
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In V for Vendetta, no one knows what happens to the people who are black-bagged... except of course for the ones who enact said black-bagging, V, who escaped, and Evey, who was put into a fake interrogation by V.
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Fullmetal Alchemist has the Fifth Laboratory, where the government's human experimentation takes place.
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Half-Life 2: Hellhole Prison Nova Prospekt combines this with Human Resources, Brainwashed and Slave Mooks.
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The house where the narrator is kept in "The Yellow Wallpaper", which used to be a Bedlam House — and in a way, it's still being used as such because she is there to recover from hysteria. Needless to say, her being kept in a house (and especially her bedroom) that she thinks could be haunted does not help her.
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La Femme Nikita: Section 1 has a cell known as The White Room, where captured hostiles are routinely subject to torture, interrogation and "Cancellation".
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Class 3-E in Assassination Classroom is a downplayed example. If you are a student at Kunugigaoka Middle School, then you will move heaven and hell to not end up in 3-E. Not only is it in an old, derelict building, it's also on top of a mountain (that has to be climbed every morning). While it will not make you go insane, plenty of non-3-E students have nightmares of ending up in it, the students in it get abused by the other students, mentally tormented by the principal and usually end up on the very bottom of society. It is later explained that principal Asano has deliberately molded Class 3-E into such a place in order to motivate the rest of the students to go above and beyond to get good grades. Things get way better once that class gets the task to kill a monster that exploded the moon, though.
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In Gadget: Past as Future, the West End Hotel has Room 306 set aside for the Sensorama, and dissidents are brought here to be brainwashed by the device, being forced to endure propaganda and strange visions beamed into their heads. Few who enter this room come out with their psyche intact, often left a jibbering wreck in the process. In-game, you get a glimpse of this room near the start. At the endgame, you unwittingly end up in the room, and the Sensorama shows you a New Era Speech by dictator Paulo Orlovsky, leading to a Gainax Ending shortly afterward.
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Parodied in one episode of Animaniacs in which Warners end up in Hades, where they annoy Satan. He eventually threatens to lock them in a room of "agony worse than all others", which turns out to be "whiny protest songs from The '60s".
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SCP Foundation: SCP-231 is held in a room where it must be subjected to Procedure 110-Montauk once every 24 hours, to prevent mass casualties associated with Fetus Terrible events. This procedure can apparently inflict as much mental stress on the Class D Personnel carrying it out as the physical suffering it entails to its subjects, which has resulted in the suicide of at least one of both groups (with one of the aforementioned events having occurred after SCP-231-3 "self-terminated"). What happens during Procedure 110-Montauk has been expunged from non-restricted records, except for a warning that it's "as horrible as you have heard" and "does involve brutal [REDACTED]".
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Discworld:
The HQ of the Cable Street Particulars in Night Watch, one of the series' darkest moments. There is a chair for people to be strapped into, there is a gully on the floor for blood to run, the walls are heavily soundproofed, and there is a single tooth left on the floor.
The Girls' Working School/Grey House in Monstrous Regiment, and some of the places the inmates get sent on work hire. When Polly mentions that the headmaster seemed all right whenever she met him in town, Tonker replies "he was good at seeming". Only a few details are given, but escapees Tonker, Lofty, and Wazzer are all Shell Shocked Veterans and none of them are over twenty.
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XCOM: Enemy Unknown has a grim fate for any incapacitated soldiers who outlive the rest of their unit and are carted off by the enemy. The survivors wake up in a painfully bright, sterile observation chamber where they can do nothing but pace or stand and await their fate, while their foes gawk from the other side of the windows. When those windows are sealed, their captors stick probes in their still-living brains to "interrogate" them by recording whatever data they can wring from the prisoner before they inevitably expire from the process. This horrific facility is... the Alien Containment Chamber in XCOM's headquarters. It's not for nothing that Central Officer Bradford refers to Dr. Vahlen, XCOM's head scientist, as "the aliens' worst nightmare".
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The Mysterious Benedict Society has the Waiting Room: so horrible that even those who have never been there cringe in fear of it. One of the main characters gets sent there, and it's just a room full of mud and insects; nevertheless, he is nearly broken by it. Later, Milligan gets captured and sent to it and it turns out to work to the party's advantage. He is able to hold his breath and sink down below the mud and then escape.
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"Sam Hall" opens with the protagonist's nephew being arrested and sent to a Room 101; the protagonist must hide that they were related.
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The Abyss in KULT, a great chasm where Demiurge's citadel once was. Only Astaroth has ever been known to get in there and return, but only barely alive. And he refuses to speak about anything inside that place.
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The Twilight Zone (1985): In "Room 2426", the titular location is a torture chamber where prisoners such as Dr. Martin Decker are taken when they have information that the State wants. In Martin's case, the location of the notebooks detailing his research into a new bacteria strain.
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Green Lantern: The Sinestro Corps have small capsules like these called fear lodges — just as Green Lanterns power their rings through willpower, Sinestros can power their rings through their own fear, so they face their own worst fear so that their rings can collect it.
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The room 1408 in the short story and movie adaptation of the same name. Somewhat unusual in that it's the room itself that does all the torture, and no one knows why.
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Overlord (2012): The black capsule is an area of Nazarick's 2nd Floor that is ruled over by the area guardian Kyouhukou. The few humans who have been tortured there were left so traumatized by the experience that they will demonstrably do absolutely anything to avoid even the possibility of going back. Even powerful members of Nazarick have mentioned that they find the place horrifying. In the seventh volume, Invaders of the Large Tomb, we see what actually happens there: victims are eaten alive by cockroaches while being constantly healed so they don't die.
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Nineteen Eighty-Four is the Trope Namer. Winston has an extreme phobia of rats, ever since he was a young boy. The culmination of his stay within the Ministry of Love, and the chilling climax of the novel's plot, involves him finally being sent to Room 101, where they have a rather unique helmet-cage apparatus waiting for him. Rats are put in one end, his head in the other, and the barriers between his face and the feral rodents are removed one by one until he surrenders; That's not to say "pleads for mercy" or "admits defeat", he has to fully surrender to Big Brother by compromising the last shred of integrity and humanity he's been clinging to, by begging them to inflict this terrible torture on his love interest, Julia, instead of himself.
Rather hilariously, Room 101 was named after a conference room at The BBC, where the book's author George Orwell had to sit through boring meetings. The inspiring room was later used as the basis for an art piece by Rachel Whiteread which filled the room with plaster, tore the walls around from it and took out the plaster left over. Seen here.
A short parody of Nineteen Eighty-Four, "1984 Has Been Unavoidably Postponed" by Alan Coren, has the Ministry of Love turn out to be short of rats — and Smith isn't scared of anything else they had to offer. He winds up actually trying to help them find a creature they can torture him with:
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Subverted in The Restaurant at the End of the Universe — the Total Perspective Vortex is a machine that causes the soul of whomever gets put into it to commit suicide by showing them how insignificant they are in the universe, but when Zaphod Beeblebrox goes in, it turns out that it merely reveals he's the most important thing in the Universe. This is later attributed to this all occurring in a universe specially created for him, where he truly was the most important person in the universe (go... read the book). At any rate, he's pissed off enough at Zarniwoop for first abducting him and then bursting his bubble about how he survived the Vortex that he eventually slugs him.
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This is the test Richard has to go through to get the Key in Neverwhere. He's shunted into an illusionary double of an Underground station and confronted by his friends and girlfriend, who tell him that he had a complete psychotic break and everything he's experienced so far is a delusion. Then it starts convincing him to kill himself...
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Exalted has the Monstrances of Celestial Portion, which is like this to the Solar Exalted. Yes, even the immortal god-kings of Creation have something that makes them scream in fright. What are they? The Deathlords traded some favors to the Yozis to create an engine that would invert a Solar Exaltation's nature and make them serve the cause of death, turning those bestowed with it into the Abyssal Exalted. If a Solar's stuck in one of them... well, it ain't pretty. Hell, a Solar can't even look at one without feeling sick.
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Batman Beyond has this in the form of the "ISO" room at the Ranch run by Dr. Wheeler in "The Last Resort". For most of the episode, there's no explanation of what it does, though we do get to see one of the former inhabitants. He sits quietly and appears to be painting, but when Terry gets close and asks him a question, he is completely unresponsive. Then you realize the tablet that he's painting with is devoid of paint and the canvas he's vigorously moving the brush across is completely blank. We eventually learn just what the ISO does when Terry meets with Chelsea, who was sent to the Ranch just for speaking out to the principal against it, who explains to him just what it actually is — total sensory deprivation used as torture and punishment.
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"Repent, Harlequin!" Said the Ticktockman has Coventry, where the Harlequin is eventually sent. By the time he returns, he's a bright-eyed, cheery... brainwashed shell.
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In the humorous BBC chat show (and later, celebrity panel show) Room 101, celebrities come on to talk about their most hated things, their pet peeves, and stuff they find annoying. They all have to make a case for their hatred, and if successful, the item is dumped into Room 101 itself.
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Roswell: The FBI Special Unit tortures Max inside "The White Room".
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Farscape: Scorpius begins the series overseeing one of these in the depths of a top-secret Gammak Base. This chamber contains his dreaded Aurora Chair, which is used to forcibly extract memories from uncooperative prisoners.
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Lake Laogai from Avatar: The Last Airbender is a place where the corrupt Dai Li do something to brainwash people. You'll find yourself completely stripped of your identity and memories, or simply a Manchurian Agent ready to kill your friends in a second.
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A That Mitchell and Webb Sound sketch depicts the problems of working in Room 102 — for example, when those noisy buggers next door are torturing someone whose worst fear is a whale with the face of his old French teacher. Also brings up the problem of what happens when people start getting wise to how Room 101 works. It seems that a suspiciously large number of people have suddenly developed a fear of sexual intimacy, and even the staff are making sure that everyone knows about their crippling phobia of beer...
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The Disturbed Ward in One Flew Over the Cuckoo's Nest subverts this. Despite the electroshock therapy and lobotomies performed there, it's actually a refuge from Nurse Ratched's tyranny, and the staff will often try to prolong a patient's time in the Disturbed Ward because Ratched is just that nasty.
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In the classic (Earth-2) Batman story "The Autobiography of Bruce Wayne", the Scarecrow crashes a wedding that Bruce is attending, and hits the congregation with fear gas, causing everyone to relive their worst fears. When Batman suddenly sees his allies (Robin, Batgirl, Commissioner Gordon, Superman) disappear before his eyes, Scarecrow actually mentions this trope by name: "So, Batman, your greatest fear, your Room 101, is autophobia!"
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Brazil, which has a lot of elements inspired by Nineteen Eighty-Four, has its own take of Room 101 at the "Information Retrieval" department, in which unlucky detainees are strapped to a chair and tortured by Jack Lint, who Gilliam described as "utterly and totally unscrupulous." The inside of a nuclear plant's chimney was used as filming location for this gargantuan torture chamber.
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The Shamer Chronicles features a room covered floor to ceiling with carvings of faces that whisper the worst things you can imagine to you — remind you of horrible things you have done or play on your insecurities until you're driven mad.
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In With Pearl and Ruby Glowing, A. Nigma has detention in Room 101, where teachers come in to abuse the students. The students also report seeing the Tatzelwurm hanging around that room.
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Fables has a group dedicated to removing the fable characters from memory. One of their punishments for fables who get out of line is what looks like a pit. Apparently being inside causes people to simply forget your existence, which for a fable can mean they simply fade from existence (Paul Bunyan was reduced to a midget after multiple trips).
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In Casper's Scare School, the students are told of a place so scary and horrible that only the screams of the monsters who enter are heard when you get near it. Subverted; in what can't be a completely unforeseen twist, the place is a garden full of beautiful flowers and trees where the banished monsters sit and drink lemonade. The place contains things that would be scary to a stereotypical monster who's used to slime and mummies and vampires et cetera.
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Black Mirror: While not used in the same sense as Nineteen Eighty-Four, the trope is absolutely played straight in the climax of "Playtest". Cooper is terrified to open the door to the access point that he is led to believe will allow the mind-altering game to end, as he thinks it will contain his greatest, deepest fear. After some coercion from Katie, he opens the door to find his suspicions were true; the game does not end, and he is subjected to his deepest personal fear: succumbing to Alzheimer's and losing his memory and his mind just like his father did. Continuing this trope, when he finally is booted from the game and returns home to America, he enters his mothers' bedroom to find to his abject terror that she has fallen into dementia in his absence. Poor Cooper actually dies abruptly in reality from the game crashing, and he spends his last living moments literally living out his worst nightmare, screaming out for his mom.
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In Downfall (2009), the main couple books a room in a hotel which is revealed to be room 101. You can imagine how well that went...
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Werewolf: The Apocalypse has the Spiral Labyrinth, which werewolves are forced to walk to turn them into Black Spiral Dancers (except in one notorious case where that didn't work, and Celeste Walks-The-Spiral-Backwards destroyed the labyrinth instead).
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Incorporated has the "Quiet Room", a torture chamber for anyone who breaks SPIGA's rules. People sent there have a tendency to not come back.
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In House at the End of the Street, the number of the prison cell Ryan ends up in is 101. It's implied that he is being tortured by the worst recollections of his life (though as a result of his own mental illness rather than outside interference)- and there are many.
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Star vs. the Forces of Evil has this in the form of Saint Olga's Reform School for Wayward Princesses. It serves as The Dreaded for quite a few episodes until Star and Marco finally go there in an attempt to bust Star's friend Princess Pony Head. They aren't wrong at all about the place. St. Olga's itself has the "Solitary Conform-ment" room, where especially troublesome students are brainwashed into obedience with a high-speed slideshow on princess etiquette.
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The Kentucky Fried Movie: In the parody "A Fistful of Yen", the villain sentences a spy with the evilest punishment imaginable: "Take him to Detroit." "No! Not Detroit! NOOOOOOOOOOOOOOOOOOO!"
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One strange example appears in the Even More Tales to Give You Goosebumps short story "The Chalk Closet". At a certain school, students who misbehave are sent to the closet, where they are forced to listen to the sound of Nails on a Blackboard for eternity.
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The "Objective Room" in That Hideous Strength is a sort of mental torture. At a glance, it's an unremarkable room, but the closer you look, especially at the pictures on the walls, the more unpleasant it gets.
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Animorphs drops some mentions of Visser 3's personal torture chamber aboard his Blade Ship. For his underlings, even death from starvation is more preferable than ending up in there.
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In The Wheel of Time, the Aes Sedai have one of these underground in Tar Valon. Thieves brave enough to try to rob the place get sent there, and are quietly released the next morning, apparently unharmed but never trying that again. We find out later what it is: you're strapped to a block and the local magic really does give you immersive hallucinations of your worst fears.
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The Attic in Dollhouse. We don't see what it actually does until late in season 2, but it's mentioned well in advance...
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In Hell House, the whole mansion qualifies, but most of all the ballroom, to the point that Ben Fischer makes it a point to try avoiding it.
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Killer7 has the Forbidden Room, which may or may not be the source of the horrific screaming and moaning heard in the building throughout the game. Eventually it's revealed to contain Kun Lan and Harman playing chess, the entities who rewrote Garcian's identity, shaping him into a pawn in their endless metaphysical battle.
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The Professionals:
Bodie and Doyle lampshade this trope after capturing a hitman who refuses to say who employed him. They take the hitman to the business of an East End gangster whose brother he killed and handcuff him to a lamppost outside. "Oh, he won't kill him... but he'll wish he had." The hitman wisely decides to talk.
In "Private Madness Public Danger", Cowley threatens to turn a drug pusher into a drug addict by forcibly injecting him with pure heroin.
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The 4 levels of dungeon in the bowels of the Red Keep from A Song of Ice and Fire were always horrible. The first level was for containing prisoners who'd committed minor offences. The second was for highborn prisoners. The third, known as the Black Cells were for containing criminals who had committed awful crimes. Ned Stark was sent there after he allegedly betrayed Joffrey. Tyrion Lannister was also sent there after allegedly killing Joffrey, before being freed by Varys. The fourth level was described by Varys as "Once a man is taken to the fourth level, he never sees the sun again, nor hears a human voice, nor breathes a breath free of agonizing pain." Once Qyburn takes control of the dungeons for his "experiments", the place gets really frightening.
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Septimus Heap: Dungeon Number One in Magyk is essentially a huge chimney-like pit full of rancid water that drowns most of its inhabitants... if they survived the fall, that is.
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One Piece:
Impel Down is known by most inmates and the public to have only five levels. However, at the very bottom of the prison lies Level 6, which the general public doesn't even know about, where society's very worst are kept pending execution and are meant to be forgotten and erased from history itself. Ironically, it's really not so bad down there compared to the rest of the place; the only torture the inmates endure is isolation.
After Buggy, Crocodile and Mihawk found the Cross Guild and Buggy is publicized as one of the Four Emperors due to a colossal misunderstanding, Crocodile and Mihawk regularly take Buggy to the "meeting room" whenever he sparks their ire with the antics of his crew. Said meeting room is in truth a torture room that happens to double as a place where Crocodile and Mihawk can privately discuss the Cross Guild's next course of action after torturing Buggy into a bloody, sobbing mess.
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In Mortasheen, the Devilbird Diablymous uses this as an attack against its opponents, feeding on the fear the act generates and leaving its victim traumatized.
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Dr. Dominiani has a whole asylum floor full of these in his domain of Ravenloft, which he uses to systematically drive people mad.
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Mage: The Ascension:
Every mage's avatar can be reincarnated, but there is also a horrid ritual to destroy avatars.
The Cauls invert a mage's avatar, turning them into one of the Nephandi, mages dedicated to corruption and destruction. For that extra nasty twist, when a Nephandi dies, it's possible for their reincarnation to be born with their past life's twisted avatar — corrupted from birth. (That ritual to destroy avatars is intended to preempt this.)
The New World Order (one of the Conventions of the Technocracy) actually has a Room 101, in direct homage to Nineteen Eighty-Four.
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In The Secret World, the Fear Nothing Foundation's downtown recruitment centre features one of these in the form of its mysterious 3rd floor. All the new recruits know and fear it, knowing that they'll only be summoned there if they've managed to piss off the counsellors; for good measure, past victims are unable or unwilling to explain what happened up there. However, it's also revealed that the counsellors are scared of it, too: it turns out that it's actually haunted by the murderous ghost of one of the punished recruits, having died over the course of brutal experimentation. It's also heavily implied that the punishments are an integral part of the FNF's indoctrination process: quite apart from the mention of disobedient recruits having left the floor "smiling like broken glass," Kinji's final letter to his parents is revealed to have been written in the aftermath of his visit to the 3rd floor.
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The now decommissioned Room 101 itself appears in the opening of The League of Extraordinary Gentlemen: The Black Dossier, which also reveal exactly where it was located; in the headquarters of MI-5, which had been the Ministry of Love during the Ingsoc years. Somewhat disappointingly, but in line with the book's deconstruction of Nineteen Eighty-Four, despite the room's near-mythical status in pop culture, it's little more than a dirty room full of mundane torture tools.
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Takanshi Kiara of hololive often makes mention of "The Usual Room", where she apparently sends KFP employees to be punished when they fail her or misbehave. When questioned by Ina about what goes on in there, Kiara revealed some punishments including walking across a floor covered in Legos barefoot and being forced to complete a one million piece jigsaw puzzle that's missing a single piece.
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Game of Thrones has Theon Greyjoy tortured, flayed, castrated and brainwashed into "Reek" inside a Medieval Room 101 underneath the Dreadfort.
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While never specifically revealed, there is some speculation that the cube in the Cube movie series is one of these. Even if you manage to live through the deadly deathtraps of death, the massive psychological horror experienced within might make you wish you hadn't... and that's only if the people outside the cube don't immediately kill you.
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Invoked in Good Eats in the episode where Alton teaches a 32-year-old man how to make breakfast himself (instead of having his mother come over and make breakfast for him.) Alton instructs his assistant, Clarence, to take the man down to Sector 12. Which turns out to be... a simulation of a grocery store, where Alton shows his pupil how to buy bacon.
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Invader Zim: Parodied in "A Room with a Moose", in which Zim opens a wormhole and had several options of places he could choose from to send his rival Dib. Out of all the unpleasant places he could send Dib and the rest of the class, he chooses... "A ROOM... WITH A MOOSE!" (His other options included a dimension of pure itching, and another of pure dookie.)
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In Carrie (and its film adaptations), Margaret has a small, cramped closet in her house filled with religious paraphernalia. She locks her daughter Carrie in there to pray for hours on end whenever she so much as takes a tiny step out of line. In the '02 version, though, Carrie manages to make it into a refuge of sorts from Margaret's insanity, hiding a stash of fashion and gossip magazines in there. The '76 version also has Carrie hiding there with Margaret's dead body as her house burns down.
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In Terminator: The Sarah Connor Chronicles, Skynet's machines operate one of these in the basement of an abandoned house in the wasteland. Precisely what happens down there is never made clear, but Derek Reese is subjected to whatever it was they did down there. Creepily enough, coming from said room is the sound of a piano playing a particular song by Chopin — the same song is playing an episode later while Cameron is ballet dancing, and later on in the series it is revealed that Allison Young, the girl whose face and identity Cameron stole, had a mother who liked that same song. Coupled with Derek Reese's familiarity of and hatred toward Cameron, there is a strong implication that she's tied to this particular instance of Room 101.
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Warhammer 40,000 brings us Commoragh, home of the Dark Eldar-a planet-sized extradimensional City 101, which is as horrible as it sounds, since its residents live on Squick.
The Inqusition keeps a well-stocked interrogation centre in the Damocles Gulf just for aliens and heretics. The most infamous is Interrogation Chamber IX.
Mankind's first contact with the Necrons occurred when a contingent of Sororitas were disintegrated to the last woman, in a place called... Sanctuary 101.
Dark Elven cities in the Fantasy Battle setting are just as bad.
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In the My Little Pony: Friendship Is Magic episode "The Cutie Map - Part 2", Starlight Glimmer locks the Mane Six (and any other dissenters to her Cult) in a room that constantly extolls her philosophy over a loudspeaker at all hours of the day. Fluttershy pretends to succumb to the abuse in order to spy on Starlight.
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Doctor Who:
There's a slight subversion with Floor 500 of Satellite 5 in "The Long Game", since everyone wants to be promoted there because "the walls are made of gold". However, it turns out to be a place of horrors, with the implication that the only people "promoted" there are troublemakers the Editor and the Mighty Jagrafess want to get rid of.
Played absolutely straight in "The God Complex", featuring a space prison resembling a hotel. Each inmate has a room which contains their worst fear, and a Minotaur-like monster that feeds on the inmates after they've been subjected to their worst fear. The Doctor thinks the Minotaur is feeding on their fear, but it is revealed the purpose of the rooms is to force the inmates to reveal their faith in someone or something to protect them, and that is what the monster manipulates and feeds on.
In "Heaven Sent", the Doctor is put to the ultimate test: He is trapped in his Confession Dial, a miniature prison, and subjected to torture for 4 billion years. What makes it especially horrifying is that it's explicitly stated in "Hell Bent" that Confession Dials aren't even supposed to be used that way.
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The Dark Tower has the Door to todash space itself in Castle Discordia. Going through it is implied to be a Fate Worse than Death by Mia.
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Severance (2022) has the Break Room, where employees are sent if they violate Lumon rules. There, they are forced to state their remorse until the lie detector determines they are telling the truth... however long that takes.
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In Unwind, the main characters are running from the law because they are scheduled for unwinding. The room where the unwinding takes place is one of these, except everyone knows exactly what goes on, just not how. We are later treated to a disturbing scene of unwinding from the victim's point of view.
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In The Burkiss Way, Room 101 contains the show's recurring irritant Eric Pode of Croydon.
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Fallen London players perusing the "Light Fingers" ambition encounter a Room 101 in the evil hellhole that is the Orphanage. It actually contains nothing worse than mops; it's a storeroom.
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In Ripples and its sequel Stirred, Level Zero of the Grand Royal Hospit of Meridian gains this reputation among the populace and the Rebellion. Mostly because it's where Will/Van and her assistants perform their medical experiments; while some of their test subjects are volunteers, the majority are imprisoned criminals and rebels.
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Bounders: While trying to find and remove the occludium tether in The Forgotten Shrine, Jasper and Mira run through a VR room that scans their minds, then plays simulations of their worst memories, all while hiding them from each other and making them feel like they're frozen in place. By the time Jasper finds the other door, Mira is in a Troubled Fetal Position on the floor.
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Unknown Armies has the Rooms of Renunciation, pocket dimensions where someone the universe doesn't like gets shut in until they come out different. How different? Depends on the room...
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The Hanoi Hilton has Room 18, where prisoners are subjected to Cold-Blooded Torture in order to break them.
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The interdimensional gateway drive used in Event Horizon is an interdimensional jump drive that can travel instantaneously to any part of the universe; unfortunately, the ship will travel through a parallel dimension analogous to Hell.
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The Pilo Family Circus has its own Funhouse, a place that even other circus employees avoid, as it's the home of the Matter Manipulator, a flesh-sculpting magician responsible for the creation of the Freakshow. Carnies are sent there as punishment for suitably unpleasant crimes, and visitors to the show that actually enter are never seen again. The only example we see of a victim of the Matter Manipulator besides the freaks is Winston the Clown, who has the flesh of his stomach ripped out and replaced with hot coals.
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Multiple episodes in season 1 of Within the Wires mention the Extensive Studies Lab, how the patients who come back from there are affected, and the various tools used there for a procedure only referred to as "carpentry".
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In BlazBlue, this is what can be described as Relius Clover's Astral Finish: Sending people to the Room 101 that is his lab.
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Babylon 5: While Sheridan is being tortured in "Intersections in Real Time", his interrogator keeps mentioning Room 17 as the place Sheridan will wind up if he doesn't start talking. Eventually, it's subverted when Sheridan's willingness to die before talking ultimately gets him sent to Room 17. Instead of getting subjected to some brutal, final torture, Sheridan is brought to an identical interrogation room and starts the whole process all over again. The ultimate horror of Room 17 is simply that the torture will never stop.
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There is a stealthy nod to the trope in the Star Trek: The Next Generation episode "Coming of Age". Wesley Crusher is taking a test to enter Starfleet Academy, and the last test is a personalized "psych test" meant to pit the candidate against their greatest fear (although Wesley is passed up for admission this time around, he is commended for handling his test well). When he is summoned for the beginning of his final test, he is summoned into "Room 101".
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The following is a list of statements referring to the current page from other pages.

 Room 101
processingCategory2
A Tortured Index
 Room 101
processingCategory2
Horror Tropes
 Room 101
processingCategory2
Speculative Fiction Tropes
 Room 101
processingCategory2
Stock Shout-Outs
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101
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Room 101