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Radiation-Induced Superpowers

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This is the tendency in fiction for exposure to nuclear radiation or other sci-fi hazards to result in a character gaining super-powers when an unpleasant death by radiation poisoning or a slow, agonizing demise by cancer would be a more likely outcome.
Don't try to tell that to anybody on the inside of the fourth wall, though.
Unsurprisingly, this trope seems to have been at its peak in the atom-crazed 1950s, when anything "atomic" was seen as cutting-edge, but is now falling out of favor as the common person's changed perception of the negative effects of radiation make it increasingly less believable as a source for superhero mutation. A few superhero characters whose backstory involved gaining powers though irradiation have since been re-written into genetic engineering being responsible to capitalize on a new area of scientific ignorance for viewers.
Godzilla movies aside, this is not a particularly common trope in Japan as, due to World War II, the Japanese are much better acquainted with the effects that atomic radiation has on human physiology than most. In fact, the Japanese Kaiju genre (which includes Godzilla) was known for highlighting the negative effects of radiation, rather than the positive effects often seen in American fiction of that era.
See also Phlebotinum du Jour (for more unlikely things that promote superpowers) and Deus ex Nukina (for more things that nuclear power can arbitrarily solve). Compare Freak Lab Accident.
A Super-Trope to Nuclear Mutant, which specifically talks about monsters created by radiation. Frequently this trope needs Radiation-Immune Mutants as a Required Secondary Power. The predecessors to this trope are Lightning Can Do Anything and Chemistry Can Do Anything; before the discovery of nuclear power, electricity and chemicals were the go-to source for magical do-anything phlebotinum. One of its successors is Quantum Mechanics Can Do Anything. Sometimes overlaps with Toxic Waste Can Do Anything when we're talking about nuclear waste. A sister trope to Atomic Superpower, which is when a being has powers over atomic radiation, though it is possible for someone to be an example of both tropes.
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In Gone, people can die from radioactivity (and some of them nearly do), but it's also a potential cause for the superpowers that some of the kids have. It's also what the local monster feeds on. Justified, because Gone takes place in an Alternate Universe where the laws of science have been rewritten.
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Wonder Woman (1942): Atomia uses nuclear radiation as part of her process of creating super-strong but essentially mindless mooks out of kidnapped humans.
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Fallout 76 introduced mutations, which are Exactly What It Says on the Tin: mutations, usually beneficial but not always particularly useful, that are gained from being overexposed to radiation. Like radiation poisoning, they can be removed with RadAway.
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The Mighty Thor: Chen Lu was turned into the Radioactive Man in a Chinese attempt to create a human weapon. Pity they didn't check if he had plans for world domination first...
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One possible Origin in Super Munchkin involves stubbing one's toe on a "super enriched radioactive block of stuff". In the base game, the Plutonium Dragon is one of the highest-level monsters.
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Parodied in Our Dumb Century; a headline from 1963 declares "Boy Bitten by Radioactive Spider Dies of Leukemia". The body of the article mentions Peter Parker as being the sixth atomic accident fatality in the last month, referring to Dr. Bruce Banner and Reed Richards and friends.
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Doom: Averted in the comic. The Doomguy is very displeased with the fact that radioactive waste is carelessly left lying around. Because now he's radioactive. And that can't be good.
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In the late 1950s and early '60s, there was a belief among certain scientists that the sudden appearance of a number of children of high intelligence was caused by the recent appearance of Strontium-90 from nuclear testing, which led to a fringe belief that humanity was undergoing an evolutionary leap. This may well have been the inspiration for the X-Men.
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Also parodied in one of the Sluggy Freelance stick-figure fillers where Torg is bitten by a radioactive animal and gains the superpower to lose his teeth and hair. Fortunately, aliens are nearby to cure him and give him real superpowers.
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Hinamori attempts to invoke this trope in Please Stop Eating The Hell Butterflies. Why? Well, she's crazy, so there's that.
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Black Canary: In the late 1960s, Black Canary gained her iconic Super-Scream due to radiation. This was later deconstructed when it was revealed several (both in-series and meta) years later that she was terminally ill due to the lingering effects of the radiation.
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The Simpsons:
The Simpsons has their own Radioactive Man, and arguably the most popular of the two.
Used to explain how Mike Scioscia can show up in "MoneyBart" despite getting radiation poisoning in "Homer at the Bat" nearly twenty years previously. Apparently, "It gave me super-managing powers. I also demagnetize credit cards."
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Scorch from Agents of S.H.I.E.L.D. is theorized to have received his powers from a nuclear accident that occurred at a Chinese power plant. However, at the same time it's mentioned that no one else involved got any powers, so in the end, the source remains ambiguous.
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Parodied in WarioWare DIY's in-universe comic about "Chimney Man" who was bitten by a radioactive chimney and gained all the powers of a chimney... well, the one power.
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The main characters of Toxic Crusaders became super-powerful (as well as hideously deformed) when they were exposed to radioactive waste in five separate unlikely accidents.
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In one episode of Back at the Barnyard, the animals believe that the Farmer is planning to sell the farm, and one of their attempts to scare off a family that they think are potential buyers is to disguise Freddy as a mutant antelope and have him shoot lasers out of his eyes, which he explains that he achieved from drinking nuclear waste. Pig attempts to debunk this trope by drinking some himself, which turns him into a HULK MASH!-Up; then the narrator tries to do the same and shrinks.
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In the Fallout: Equestria universe, as in the original Fallout franchise, the radiation in the Equestrian Wasteland causes all manner of crazy mutations in animals and ponies alike. This is actually more justified than in the original, seeing as the "radiation" originated from magical superweapons.
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Sky High (2005):
Referenced by the school nurse; "The kids who get bit by radioactive insects or fall into a vat of toxic waste, their powers usually show up the next day. Or - they die."
Will's mom mentions this when they find out he doesn't have powers.
Also by Layla and Magenta.
At the very end, Ron Wilson (Bus Driver) falls into a vat of toxic waste and receives powers.
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The Flash:
Jay Garrick, the Golden Age Flash, gained his powers when he accidentally inhaled fumes of heavy water, a rare non-radiation-based version of nuclear superpowers.
A minor recurring adversary is named Fallout, a former blue-collar worker who was hired to do work on a nuclear power plant, fell into the reactor, and emerged with translucent green skin and radioactive powers that caused him to inadvertently kill his wife and son. After Flash apprehended him, he agreed to act as a living power source for the prison in which he was incarcerated as penance.
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Subverted humorously in the Aqua Teen Hunger Force episode "Super Hero" when Master Shake exposes himself to toxic waste that, instead of giving him superpowers, causes him to slowly melt.
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This may or may not be the cause of the mutation of every last citizen of Alpha Complex in Paranoia.
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In one episode of The Flamin' Thongs, Holden gains temporary insect-based superpowers from eating radioactive cockroaches.
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One of the main villains on Captain Planet and the Planeteers was Duke Nukem (no relation to the video game character of the same name), who had been transformed by radiation into a mutant that feeds on nuclear waste and radioactivity.
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Discussed in Freeman's Mind, with Freeman pointing out that the chances of gaining a beneficial mutation from being exposed to radiation were astronomical, and even if you did get one, you'd still have radiation poisoning.
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In Freefall, not only is the ship run on fusion, dislike of it is allowed to run to rampant paranoia.
Referenced later on here:
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In Heroes Unlimited, Mutants and Experiments can potentially get their powers through exposure to radiation. Even prolonged exposure to small amounts of radiation can do the trick, as shown with the supervillain Gold Falcon in the "One Dam Thing" adventure: spend enough time standing next to a leaky microwave and you too can gain the power to fly and shoot blasts of electricity!
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Parodied in the Earthworm Jim cartoon for one episode where Jim, attempting to get superpowers to replace the weak super suit copy he was stuck with, used comic book methods. His efforts include getting trapped in a nuclear reactor, which gives him a glow-in-the-dark rash, and being bitten by a radioactive flea, which causes him to gain out-of-control leaping powers and grow flea legs from his head.
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Whateley Universe: While rare, exposure to radiation can indeed cause people to manifest superpowers, part of a larger catch-all category of supers known as 'Batson Factors'. Unreliable Narrator Mephisto mentions that in the 1950s, there were indeed many superheroes and supervillains whose powers came from nuclear radiation (with many of them even having 'Atomic' in their codenames), but most of them had short careers, as many lacked protection from the long-term effects of radiation, meaning that the combination of radiation poisoning and cancer soon caught up with them.
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Sleeper: Genocide Jones had a job at a "weird government research plant" that features a pair of iconic nuclear cooling towers. Being a loner, he took his lunch in an isolated area of the plant, prominently marked with radiation warnings. He somehow doesn't notice he's getting bigger and stronger.
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In The Fall, Louise, while already having magic before arriving in the Mojave. begins to experience some changes to it due to the radiation of the world.
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Elementary references the comic-book usage of this trope while investigating a crime adjacent to a superhero comics publisher. Sherlock pours scorn on it, naturally ("in what universe are these characters not all dead from cancer?"), and when asked how he got so good at his job, he deadpans:
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Listen to the theme-song for the old Spider-Man (1967) animated series: "Is he strong? Listen, bud, he's got radioactive blood!" In real life, people with radioactive blood aren't particularly strong.
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In The Adventures of Dr. McNinja, "crazy space radiation" seems to do a lot of crazy things, like grant superintelligence to dinosaurs and create "NASAGHASTS", malevolent astronaut ghosts. It's not surprising, considering how the comic is influenced by Teenage Mutant Ninja Turtles and other '80s nostalgia full of this trope.
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Super Fuzz is an Italian comedy movie set and filmed in Miami where policeman Dave Speed gains all kinds of superpowers (invulnerability, super speed and reflexes, precognitive abilities, telekinesis and so on) by being exposed to the radiations of a nuclear missile test.
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The Big Bang Theory: Discussed in the episode "The Alien Parasite Hypothesis":
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In X-Men: First Class, Sebastian Shaw believes that mutants are the "Children of the Atom" and believes all mutants are immune to radiation because of this. This is why he plans to turn the Cold War nuclear, believing that the radiation will wipe humanity out but spare mutants.
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In Batman: The Brave and the Bold, B'wana Beast gets his powers from drinking water contaminated with nuclear waste (in the comics, it comes from a special elixir and helmet).
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Hothouse: Traveling from the Earth to the Moon exposes you to radiation, causing humans to mutate and begin transforming into flymen.
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Quantum and Woody: The titular pair got their powers after they were accidentally bombarded with quantum energy.
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Fallout: New Vegas reverts many of the Atom Punk elements, but you can make your character stronger from absorbing radiation with the Atomic! perk (though you still get sick from it).
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The final mission of Saints Row: The Third DLC "The Trouble with Clones" has the Boss temporarily gain superpowers after Jimmy gives them irradiated Saints Flow.
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The New 52 reboot embraces this, turning "the Firestorm Protocols" into an extended nuclear arms race metaphor.
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Ruins: The Marvel Comics Elseworld Miniseries subverts this repeatedly. In its vision of a darker, bleaker Marvel Universe, it imagines the "realistic" effects that the numerous radiation-fueled Freak Lab Accidents that gave many of their comic book superheroes their powers (gamma radiation bursts, "cosmic" rays, irradiated spider-bites, etc.) could have — specifically, painful disfigurements and horrible deaths. However, the series often leaves in the other unrealistic elements; for instance, Bruce Banner becomes a mass of tumors, but still violates conservation of mass in doing so.
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Many of the Marvel Universe origins are given a kind-of explanation in Earth X, in that certain people have the ability to gain superpowers. What those powers are is determined by how they get them, but because of this innate "spark", they do indeed gain abilities from things that would kill people without it.
This is roughly the same rationalization behind the "metagene" in The DCU.
In the mainstream continuity, radiation-based origins have been explained as genetic experiments by the Celestials that were triggered by radiation.
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Apparently the most popular way to gain powers in Heroine Chic:
Superhero Khatie was hit by a "radioactive rubber ball," which gave her the power to store up kinetic energy when she bounces.
Amp was bitten by a radioactive eel, granting her the ability to generate electricity.
Dyna's husband Gareth, who operated under the superhero name Big Kahuna in the 1990's, "ate some bad poke" after atomic testing done in Pacific Ocean. The radioactive seafood he consumed gave him the ability to shoot force beams out of his eyes.
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Late in StarCraft II: Heart of the Swarm, during a bonus mission to upgrade the Ultralisk, Mengsk orders his men to drop an experimental nuclear weapon over a group of Ultralisks. The Ultralisks start suffering radiation poisoning, but Abathur, thinking quickly, alters the genetic sequences of the Ultralisks to allow them to assimilate the radioactive particles and use them to change further. The result? The Ultralisks turn into Torrasques, beasts capable of resurrecting themselves when killed. Nice job there, Mengsk!
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In The Powerpuff Girls (2016), one villain is a gnat who was bitten by a radioactive man. He then turned into an abnormally large, lumpy-looking humanoid/gnat hybrid.
 Radiation-Induced Superpowers / int_671dadb5
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Most of the Leader's schemes revolve around trying to mutate humanity with gamma radiation, most recently in Fall of the Hulks.
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Played a bit more logically in Batman Beyond: The radiation that turned Derek Powers, the Big Bad, into the super-powered Blight was actually therapy for a dose of his own experimental nerve gas. Somehow, their combined effects turned him into a glowing green skeleton, possessing explicitly radiation-based superpowers and weaknesses, with a half-life of one season.
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In the case of She-Hulk, apparently deadly radiation can turn you into a 6'7" green supermodel who can bench a train. However, She-Hulk didn't get her powers from direct exposure to radiation, but rather a transfusion of radioactive blood from Bruce Banner, her cousin...
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Fallout 3 became a straighter example of this trope, with the player character able to gain healing from being irradiated. The game also made the pre-war world a lot more Atom Punk than earlier games, with nuclear-powered cars.
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In Fallout 4 the player can choose a dialogue option joking that they'll run through a heavily irradiated area in their underwear in the hopes of gaining superpowers. Doctor Amari feels the need to remind the Sole Survivor that this would only kill them, though there may be a few players out there that did so anyway. Though you can get a perk that effectively lets you metabolize radiation, lowering your radiation level while regenerating your health.
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Both the Superman villain Neutron and the Supergirl villain Reactron have the ability to control and project nuclear radiation.
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Practically everything in Rocket Age is powered by Radium. Despite being retro sci-fi, it is not advisable to get too close to the glowing rocks. However, radiation may have a link to psychic powers.
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In Our Friend Power 5, Hyuk gets struck with gamma rays in a laboratory accident, and winds up with Psychic Powers, such as the power to shoot energy from his hands.
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The Fallout series started off as a deconstruction of the 1950s "atom craze". The nuclear war gave rise to giant insects and rats, and a few (un)lucky humans exposed to it without dying became ghouls, who live for centuries but many of them have lost their humanity. Meanwhile the player character being exposed to radiation will only result in radiation sickness (though it is easy to treat). However, ghouls are healed by radiation.
Fallout 3 became a straighter example of this trope, with the player character able to gain healing from being irradiated. The game also made the pre-war world a lot more Atom Punk than earlier games, with nuclear-powered cars.
Fallout: New Vegas reverts many of the Atom Punk elements, but you can make your character stronger from absorbing radiation with the Atomic! perk (though you still get sick from it).
In Fallout 4 the player can choose a dialogue option joking that they'll run through a heavily irradiated area in their underwear in the hopes of gaining superpowers. Doctor Amari feels the need to remind the Sole Survivor that this would only kill them, though there may be a few players out there that did so anyway. Though you can get a perk that effectively lets you metabolize radiation, lowering your radiation level while regenerating your health.
Fallout 76 introduced mutations, which are Exactly What It Says on the Tin: mutations, usually beneficial but not always particularly useful, that are gained from being overexposed to radiation. Like radiation poisoning, they can be removed with RadAway.
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Promethean: The Created has the Zeka, named after the Russian gulag prisoners who worked the uranium mines. They're the result of several demiurges who exposed corpses to nuclear power, triggering the Azoth to reanimate them. They may have the worst luck of any Promethean - they're living fallout, doomed to exist only in radiation-filled hellholes. And if they pull off the Great Work and become human? They have an excellent chance of dying from radiation poisoning thanks to their innate radioactive contamination. No wonder so many of them turn to The Dark Side.
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Wing Commander: In the Confederation Handbook, mutations from cosmic radiation are said to be the cause of Pilgrim powers, though not in the short term as often depicted by this trope, taking multiple generations.
 Radiation-Induced Superpowers / int_7a0eb880
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The Simpsons: In one issue, a meltdown at the Springfield Nuclear Plant triggers a Mass Super-Empowering Event that affects almost everyone in town except for Bart. Parodied when Bart immediately and sincerely declares that gaining superpowers is the obvious, logical outcome to radiation exposure.
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Immortal Hulk postulates a parallel explanation: in the Marvel universe, gamma radiation is equal parts science and magic. It works like science expects it to work... until it doesn't, until it starts turning people into metaphors for their psychology, because a multiversal cosmic horror that's the antithesis of the omnibenevolent One Above All hates all creation and wants to corrupt it until all is destroyed.
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Some superheroes (and villains) in Champions received their powers from nuclear radiation or being descended from people exposed to radiation. Also, older editions of the game allowed players to completely redesign their character if the plot would allow for it and the GM agreed — the rules refer to this as a 'Radiation Accident', even if nuclear energy had nothing to do with it.
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Averted in one of the New Teen Titans shorts, showing a blooper reel from an in-universe PSA in which the Titans have to say the line "No matter what people tell you, gamma rays will not give you superpowers."
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In Single Parents Night, it's believed that Tails having the ability to fly has to do with him being found by a chemical plant.
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The titular Yars of Yars' Revenge, as revealed in the included comic, used to be ordinary houseflies until the rocket they stowed away on was destroyed by the Qotile. The flies were the only survivors, and the radioactive wreckage mutated them into Insectoids capable of unaided interplanetary flight and converting any matter into powerful Energy Weapons by eating it.
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Superman:
Averted in the comics Post-Crisis (albeit played straight elsewhere in The DCU). The chronic health problems that plague Lex Luthor in both his comic book and cartoon series appearances are a result of exposure to the Kryptonite ring he wore for quite some time. While it certainly hurts Superman very quickly, having it around you for years will have the same effect any kind of radiation will.
In Krypton No More, Superman villain's Radion's powers were caused by a nuclear accident at an atomic power plant. He possesses radioactive powers and the ability to cause atomic decay.
Both the Superman villain Neutron and the Supergirl villain Reactron have the ability to control and project nuclear radiation.
Deconstructed in the origin of the Cyborg Superman. In a pastiche of the Fantastic Four, a space shuttle crew is exposed to cosmic radiation but suffer vastly detrimental effects. Two are killed immediately and resurrected in painful or dangerous forms, eventually leading them to suicide, and one is nearly drawn into an alternate dimension. The fourth member of the crew, Hank Henshaw, suffers an accelerated radiation poisoning which rots away his body. However, Henshaw's mind quickly returns to life with technopathic abilities (and rampaging sociopathy).
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This is roughly the same rationalization behind the "metagene" in The DCU.
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Dark Secrets of Garry's Mod: "Super Boss" is a made-up teaser in which Boss gains superpowers from eating a radioactive bean pottage. It got deleted but it is reuploaded into a compilation.
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Used in Buzz Lightyear of Star Command, as a G Rated Fantastic Drug. Mira gets addicted to phasing through nuclear cores, which ups her power and speed to somewhere in the range of Superman and The Flash. It's also a subversion, as she suffers radiation withdrawal, complete with unkempt hair, dark circles under her eyes, and general creepiness.
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In Krypton No More, Superman villain's Radion's powers were caused by a nuclear accident at an atomic power plant. He possesses radioactive powers and the ability to cause atomic decay.
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Parodied in The Fairly OddParents!. The Crimson Chin's Superhero Origin has him bitten on the chin by a radioactive celebrity.
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In Patalliro!, Patalliro tries to hatch a "super duck" by irradiating a duck egg, but what hatches is just a rather large duck.
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In The Amazing World of Gumball episode "The Crew", Gumball and Darwin are convinced that in order to join a gang of senior citizens they need to look old, so they try to get wrinkles by wallowing in nuclear waste. They don't get wrinkles, but Gumball gains the power of telepathy and Darwin becomes magnetic.
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In the SuperMarioLogan video "Black Yoshi's SuperPowers!", Black Yoshi finds some green, glowing fried chicken in the garbage and eats it, which causes him to get Eye Beams and Super-Speed.
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The Zebesian Space Pirates in Metroid Prime use Phazon, a radioactive substance, to create elite troops. If the player reads the Pirate Data entries from your scan visor, it is learned that some of those exposed to Phazon radiation go insane and attack their allies.
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In JoJo's Bizarre Adventure, it is eventually revealed that the apparently mystical Stands were somehow created by an ancient artifact made from a radioactive meteorite.
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If the Emperor Had a Text-to-Speech Device pokes fun at the idea of radiation causing random mutations when Uriah is accusing the Emperor of encouraging the Imperium's worship of himself:
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Wonder Weenies has the main characters gain their powers due to a malfunctioning nuclear hot dog cooker, the Frank-N-Fryer.
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Spider-Man:
Spidey himself famously acquired his powers after being bitten by a radioactive spider. Just about every adaptation since has veered away from this, making it a genetically modified test subject instead.
Many of Spidey's classic foes gained their powers from some type of radiation accident as well, but special mention goes to Doctor Octopus. Not only was the good doctor an actual atomic scientist who would later use this knowledge in several of his evil schemes, but in one retelling of his origin, Spider-Man/Doctor Octopus: Year One, he considers himself and Spider-Man to be twins of their "mother", nuclear power. Ock generally seems to take this trope's title literally.
Turns out that irradiating the beehive you were studying will mutate the insects and cause them to eat you alive! Don't worry, though — you'll live on in their new-formed Hive Mind, your new body composed of bones and bees! Now you have to use this new power to go into supervillainy, fighting Spider-Man as the sinister Swarm! At least, if you happen to be a Nazi scientist in the Marvel Universe.
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The Incredible Hulk: The Hulk, the Leader, the Abomination, and all the other people mutated by gamma radiation.
In the case of She-Hulk, apparently deadly radiation can turn you into a 6'7" green supermodel who can bench a train. However, She-Hulk didn't get her powers from direct exposure to radiation, but rather a transfusion of radioactive blood from Bruce Banner, her cousin...
And they're only the most famous. The Hulk comics have seen a whole horde of people mutated by gamma radiation over the years.
Most of the Leader's schemes revolve around trying to mutate humanity with gamma radiation, most recently in Fall of the Hulks.
There's also the Red Hulk, who even absorbs radiation. Combining gamma radiation and cosmic power will let you do that, apparently.
Subverted when Rick Jones exposes himself to gamma rays to try to develop Hulk-like powers and gets cancer instead. He gets better, though. Later, he gets turned into a gamma-powered superhuman for real.
As Science Marched On and it became increasingly difficult for readers to accept this trope straight, Bruce and other Gamma mutates were retconned to have inherited a special genetic trigger. Bruce, his relatives, and a few others are all descendants of the original carrier. The guy who discovered said trigger found a way to copy it and used it to become a Gamma mutate too.
Immortal Hulk postulates a parallel explanation: in the Marvel universe, gamma radiation is equal parts science and magic. It works like science expects it to work... until it doesn't, until it starts turning people into metaphors for their psychology, because a multiversal cosmic horror that's the antithesis of the omnibenevolent One Above All hates all creation and wants to corrupt it until all is destroyed.
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In Perry Rhodan, the first Mutant Corps consisted almost solely of individuals endowed with various Psychic Powers due to their parents' exposure to radiation — including, though not limited to, the nuclear bombs dropped on Hiroshima and Nagasaki.
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Taking place in the 1970s as imagined by the 1940s, characters in Space Age have a very casual attitude about radiation exposure, both from their own nuclear-powered gadgets, and from the radioactive ore they've found on the planet Kepler-16. The latter nearly kills two characters before giving them super-powers.
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Chiasmata: Misanthrope, a friendly, social girl who is so radioactive that can't even be in the same room as anyone without some serious detriments to their health.
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In Teeth, it's implied that Dawn's "power" is a side-effect of living next door to a nuclear power plant (thanks to said power plant being the subject of many, many, MANY shots). More mundanely, Dawn's mother's illness is implied to have something to do with this too.
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Danny Phantom uses this trope a little lightly; Danny is irradiated by ecto energy that alters his genetic structure.
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Legion of Super-Heroes: Reoccurring enemy Radiation Roy has this marked in his name. His ability to emit paralyzing radiation was paid for with an inheritance he gained so he could specifically join the Legion. Roy was rejected because his uncontrolled powers could harm the other Legionnaires, though a later retcon states that he was also rejected because Saturn Girl's mental profile revealed he had a number of psychotic tendencies. Saturn Girl was supposedly so disturbed by what she saw in Roy's mind that she couldn't sleep for two nights. As he got older, it became clear Roy's powers were having an effect on his body when he came back bald. When Geoff Johns brought the original Legion's continuity back, Roy had to wear a full-body containment suit because his powers were causing him to grow giant tumors and his teeth were falling out. Though for some reason he had hair again.
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SpongeBob SquarePants has the Atomic Flounder, a retired villain originally for a one-off gag. He later appears in a Show Within a Show episode during his prime. His first appearance follows the more common use of the trope, with atomic breath; however, the second also brings some Body Horror into the mix.
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Team Fortress 2 invokes this trope (parodiously, as always) with the 'Bonk!' energy drink for the Scout. As the advertisement tells us, "Bonk! is fulla radiation, which as we all know is pretty great for giving people superpowers."
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Super Filete: Filete and Tocino got their powers by eating radioactive steak and bacon, respectively.
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In Superman IV: The Quest for Peace, Lex Luthor says that nuclear power mixed with genetic material will create a being more powerful than Superman.
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Uncyclopedia's People's Nuclear Program article, affectionately referred to as "the 'What Can We Put a Nuclear Reactor into Today?' program"; a USSR project that resulted in a super-powered assault rifle, sword, child, and toaster.
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InvestiGators subverts this with the Were-Helicopter Dr. Hardbones, who specifies that it was a rabid helicopter that bit him, not a radioactive one.
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Firestorm (DC Comics):
Firestorm's origin involves terrorists leaving Ronnie Raymond and Martin Stein to die when they blow up the latter's nuclear plant. The explosion ends up fusing them into a superpowered being instead. Later averted when Stein is diagnosed with an inoperable brain tumor as a result of being one half of the nuclear man midway through the second series.
Also true of his Distaff Counterpart Firehawk, his Russian foe-turned-ally Pozhar, and several of his recurring villains.
The New 52 reboot embraces this, turning "the Firestorm Protocols" into an extended nuclear arms race metaphor.
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Daredevil: When Matt Murdock got toxic waste spilled on him, he gained superpowers but also got blinded. Lampshaded in one of the comics; when the empowering accident is discussed, a character points out, "You know what would happen to me if I got hit in the face with a radioactive isotope? I would get leukemia and die."
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In Amazing Fantasy, Izuku is bitten by a radioactive spider like his mentor, Peter Parker. As a result, he gives off intense amounts of unknown radiation while using his Intangibility powers that fry any electronics he passes through. This also exposes the true nature of his abilities to U.A., who decides to question him about them not long after he gets his admission letter.
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Sonic the Hedgehog (Archie Comics): Knuckles the Echidna had his egg irradiated with Chaos Energy from the Master Emerald by his father Locke (himself self-subjected to radiation and genetic testing), granting him powers and abilities far beyond even his own lineage had as the crystal's guardian. Likewise, his ancestor Dimitri, aka Enerjak, became a near-god from excess radiation siphoned off of the Master Emerald. In fact, if a character doesn't have a natural affinity for powering up with the Chaos Emeralds (like Sonic or Shadow), any Chaos-imbued powers they gain are usually a result of this trope.
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In The Million Dollar Duck, the titular duck gains the ability to lay golden eggs by escaping from the animal lab and wandering into the radiology lab, where she hops into a machine and into a beam of radiation.
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In Deadlands: Hell on Earth, being exposed to supernaturally charged radiation could potentially give useful mutations (like an extra mouth that consumes the life essence of all that die near you), or it could give you a horrible deformity (like an extra mouth that never SHUTS up), or it could just kill you. Then, there are the rad-priests called Doomsayers, who prove that, if you love radiation enough, it just might return the favor.
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Cosmo Cat: The protagonist of the short-lived series got his powers when he worked at a munitions plant. He accidentally tripped and dropped a U-235 bomb he was carrying.
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d20 Modern sourcebooks d20 Future and d20 Apocalypse provide rules for giving characters mutations, with the soft-SF Handwave of radiation or other mutagens. d20 Modern is explicitly a "cinematic" game, meaning everything can be justified by Rule of Cool, meaning this trope is meant to be in full play. However, the two books also offer sidebars discussing a sliding scale of more realistic treatments of mutation and exposure to radiation.
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Action Fish plays with this trope. The protagonist, Fishbo, turns into Action Fish due to the radioactive waste poured into the ocean, however, his friends and family perish when the radioactive waste gets dumped into the ocean. Also, you can gain bonus points by collecting radioactive waste buckets.
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Modern Problems: Max is given superpowers (specifically, telekinesis) when a nuclear waste truck dumps nuclear waste on him and his car.
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Viciously averted in GURPS; too much radiation will cause all sorts of horrible things to happen to you even if you successfully make a save against the effect. In fact, radiation damage causes a build up of genetic damage that is incurable without special powers or advanced technology. However, "weird radiation" can result in powers.
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In We Happy Restaurant, you feed your customers radioactive food which lets them mutate while boasting about how environment-friendly your radiator is. And they completely fall for it.
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Nuclear Throne:
Radiation is the name of the game: all enemies drop it except for the IDPD before the third loop, and it functions as the game's exp system, with you being able to select a new mutation with each level. All of the mutations are beneficial to some degree. In addition, the Ultra weapons all drain radiation on use but are insanely powerful as a result. Horror takes it even further by being literally living radiation and having the ability to blast enemies with it.
Melting zig-zags this a bit. Radiation exposure didn't mutate him like the other characters, but instead forced his skin to start melting off of his body, making every living second agonizing. In-game, he's a One-Hit-Point Wonder, but also gains more rads from all sources - thus why he isn't a complete aversion.
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Played straight and parodied in the Family Guy episode "Family Guy Viewer Mail #1". The Griffins are exposed to radioactive waste, and each gain separate powers (Stewie gets telekinesis, Brian gets super-speed, Chris gets pyrokinesis, Peter gets shapeshifting, Lois gets super-strength, and Meg can... extend and retract her fingernails). They proceed to wreak havoc in Quahog, and in an attempt to gain superpowers to stop them, Mayor Adam West rolls around in radioactive waste:
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Fantastic Four:
The titular Four gained their abilities from "cosmic radiation" that their spacecraft's shields couldn't keep out. Villains like the U-Foes, the Red Ghost, Red Hulk, and Feilong copied them to gain their own powers.
Immortal Hulk positions cosmic rays as gamma's equal and opposite, straddling the line between science and magic in the same way. Wasp, by the same author explains that cosmic rays are a mixture of gamma, X-rays, and kirbons, a primal building block of reality.
The Puppet Master has dolls made out of 'radioactive clay' which allow him to control the person who the doll resembles. This was clearly inspired by Hollywood Voodoo, making it arguably the most blatant example of "radiation = magic" in comics. However, this was later retconned so that the dolls' properties are due to actual magic, instead of their negligible radioactivity.
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The Mighty: Alpha One was once a normal sailor who had ended up floating in irritated waters for hours after testing an atom bomb. It took place in 1952. Or at least that's the official story. He's actually a centuries-old Human Alien.
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Two recurring villains of The Mask: The Animated Series got powers this way. First, they were two stupid teenagers that decided to get superpowers. They go to the nuclear power plant, get radioactive - and realize they forgot to bring a bug to bite them just before passing out by poisoning. As the ambulance is taking them away, an accident causes one to crash into a putty shop (turning him into the shapeshifting Putty Thing) and another into an aquarium (turning him into the harmless Fish Guy). Fish Guy didn't get anything good out of the deal either; even as a fish he still couldn't swim.
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Zack Snyder's Justice League: Upon landing in the Ghost City of Pozharnov, Russia, the New God Steppenwolf sets up his lair there. The town has been abandoned because of a nuclear meltdown ala Chernobyl, and upon feeling the radiations, Steppenwolf says "It's toxic. That's good."
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Dolphin Trilogy: Early in her pregnancy, John's mother Raye is briefly exposed to nuclear radiation, giving her son more efficient lungs than other humans and glands in his skin that cause him to be covered in oil when he's in the water for a certain period of time. These adaptations allow him to survive life with the dolphins.
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The premise of the Nuclear type introduced in Pokémon Uranium.
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Embraced lovingly by every edition of Gamma World and its sister setting, Metamorphosis Alpha.
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City of Heroes explicitly uses this; by taking a mission to save the local nuclear reactor from villains, you mutate and get to re-organize your powers. Inverted with the Radiation powersets, which use green radiation to weaken and harm enemies and buff allies (likely based on a bit of Rule of Cool in regard to real world radiotherapy). The signature character Positron is well-known for his radiation powers, and (until recently) having to wear a containment suit of Powered Armor all the time so he doesn't blow up.
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The UEF in Supreme Commander. They have two types of nuclear reactor, and one of their experimental weapons fires mini-nukes. Their Hero Unit can be armed with a backpack missile silo which can build one each nuke and counter-missile.
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Strontium Dog: Johnny Alpha gained the ability to read minds, see through solid objects, and emit alpha rays from his eyes following strontium-90 fallout during a nuclear war. However, most other mutants in the series are merely disfigured.
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Captain Atom: Taken to its uttermost extreme, as the titular character, rather than merely being irradiated, was actually vaporized by being at ground zero of a thermonuclear explosion. His mind or soul was somehow able to form a new body for itself, one with superpowers. In the Post-Crisis remake of the character, the writers explained this as an effect of the extra-dimensional substance in which he was encased at the time of the blast.
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The following is a list of statements referring to the current page from other pages.

 Radiation-Induced Superpowers
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Artistic License – Biology
 Radiation-Induced Superpowers
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Discredited Trope
 Radiation-Induced Superpowers
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Make My Index Live!
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Power
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Speculative Fiction Tropes
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Superhero Tropes
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Transformation Causes
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